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EC number: 281-589-3
CAS number: 83969-12-4
Mutagenicity without metabolic activation,
∑M = sum of mutants in all 5 dishes
NPC = no. of planted cells
MF/1E+05 cells = mutation frequency
Mt/Msc = no.
of mutants in test conc. vs no. of mutants in solvent control
Mutagenicity with metabolic
activation, 3h treatment
Mutagenicity with metabolic activation, 3h
The test item was assayed for mutagenicity in in vitro mammalian cell
gene mutation test, according to OECD guideline 476. Chinese hamster V79
lung fibroblasts were used for testing; test substance was dissolved in
DMEM. Cytotoxicity tests without as well as with metabolic activation
were performed to provide the optimal dose range for the mutagenicity
study. Concentrations used for cytotoxicity testing were in the range of
0.0005 – 2.0 mg/mL.
On the basis of cytotoxicity testing results, six concentrations were
chosen for the mutagenicity experiment without metabolic activation,
i.e. 0.01, 0.015, 0.020, 0.025, 0.03 and 0.04 mg/mL. Four concentrations
0.02, 0.05, 0.1 and 0.2 mg/mL were used for the experiment with
metabolic activation. The concentration of 0.2 mg/mL was completely
toxic and in the concentration 0.1 mg/mL insufficient number of cells
was transferred for further cultivation. An additional experiment was
performed with the concentration of 0.075 mg/mL and the concentration
0.1 mg/mL was repeated to get four analysable concentrations according
The metabolic activation was performed by S9 fraction of rat liver
homogenate and mixture of cofactors. Concurrent positive controls
verified the sensitivity of the assay and the metabolising activity of
the liver preparations. Average mutant colony counts for the vehicle
controls were within the current historical control range for the
Under the experimental conditions, test substance was non-mutagenic for
V79 cells with and without metabolic activation.
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