Reaction products of diazotized Dodecyl Aniline coupled with 8-acetylamine-1-hydroxynaphthalen-3,6-disulphonic Acid, sodium salts

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates:

EC₅₀ (Daphnia magna, 48 h) > 4.90 mg/L mg/L (geom. mean of measured conc. corresponding to nominal 100 mg/L)

Toxicity to aquatic plants other than algae:

ErC50 (Lemna minor, 7d,frond number)> 27.81 mg/L (measured initial values, corresponding to 100 mg/L nominal)

Additional information

Short-term toxicity to invertebrate

In order to determine the acute toxicity of the test substance to Daphnia magna, a static test was performed according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation (EC) no. 440/2008.

The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.At the beginning and at the end of the test, the content of the test item in the test solutions was determined using aphotometricmethod. Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

Three concentrations showed toxicity between 5 and 40 % immobilisation. None of the animals was immobilised in the blank control. The lowest treatment is not required for evaluation, as no immobilization has already occurred in the second lowest treatment. The recovery of the measured initial concentration (stability) is with 61 - 96% within the acceptable range.

Therefore the biological results were based on the geometric mean of the measured concentrations. The EC50 values are above the solubility limit and are therefore given as a range.

Aquatic Plants toxicity

This study was performed in order to evaluate the toxicity of tehe test item towardsLemna minorfollowing the OECD Guideline 221 (2006).The study was performed using 5 concentrations ranging from 1.0 to 100 mg/L (nominal concentration). Incubation time (test systemLemna minor) was 7 days. The frond number of each replicate was determined at the beginning, at day 2 and 5 during the test and at the end of the experiment. Additionally, the dry mass of 12 representative fronds was determined at the beginning of the experiment. At the end of the experiment the dry mass of each replicate was determined. Growth rate µ and the yield were determined from the frond number and the drymass at the respective observation times.At the start and at the end of the test, the content of the test item in the test solutions was determined using Photometer.The 7d-EC50s of 3,5-Dichlorophenol (1,3-Dichloro-5-hydroxybenzene, C₆H₄Cl₂O, CAS-No. 591-35-5)were determined in a separate reference test

Significant inhibition of plant growth was observed at the following concentrations: 32 - 100 mg/L (nominal). The measured concentrations lay between 20 % and 28 % of the nominal concentrations at the beginning of the test and between 6 % and 13 % of the nominal concentrations at the end of the test. The measured concentrations decrease during the test although the test item was stable during validation. The decrease of the measured concentration of test item can be explained by adsorption and/or uptake of the test item; this is indicated by the observation that some parts of the plant showed a red coloration. Part of the test item was removed with the plants from the test system before the analytical determination. Therefore, the measured initial values are used for statistical evaluation. This procedure is analogous to the OECD Guideline 201 (§40) of the algae test.

All EC₅₀values (except from yield dry mass) are above the solubility limit and are therefore given as a range.

The EC₅₀of positive control value of the growth rate lay within the desired range of 1.7 -5.7 mg/L, mentioned in the paper “OECD Lemna Growth Inhibition Test, Development and ring testing of draft OECD test guideline”.

 

JUSTIFICATION FOR CLASSIFICATION OR NON-CLASSIFICATION

According to the CLP Regulation (EC) No. 1272/2008, Part 4: Environmental Hazards, substances can be classified as hazardous to the aquatic environment when the following criteria are met:

A) Acute (short-term) aquatic hazard Category Acute 1: 96-hour LC₅₀ (fish) and/or 48-hour EC₅₀ (crustacea) and/or 72- or 96-hour E_rC₅₀ (algae or other aquatic plants) ≤ 1 mg/l.

B) Long-term aquatic hazard (iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

- Category Chronic 1: 96-hour LC₅₀ (fish) and/or 48-hour EC₅₀ (crustacea) and/or 72- or 96-hour E_rC₅₀ (algae or other aquatic plants) ≤ 1 mg/l;

- Category Chronic 2: 96-hour LC₅₀ (fish) and/or 48-hour EC₅₀ (crustacea) and/or 72- or 96-hour E_rC₅₀ (algae or other aquatic plants)> 1 to ≤10 mg/l;

- Category Chronic 3: 96-hour LC₅₀ (fish) and/or 48-hour EC₅₀ (crustacea) and/or 72- or 96-hour E_rC₅₀ (algae or other aquatic plants) > 10 to ≤ 100 mg/l.

 

The substance is not rapidly degradable and the acute short-term tests to invertebrates and plants fixed effect levels which did not meet the criteria for any hazard Categories according to the CLP Regulation (EC) No. 1272/2008. Therefore, the substance is not classified for aquatic toxicity based on the available data.