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EC number: 232-800-2
CAS number: 9025-57-4
The acute toxicity of Xylanase, PPQ 30189 to Daphnia magna was assessed
under static exposure conditions. The active component of the test
substance is an enzyme, which was known to be pH sensitive with reduced
enzymatic activity under alkaline conditions. In order to maintain the
stability of the enzyme the dilution medium used in the study was
adjusted to pH 6 before use.
The study was conducted in accordance with EC Methods for Determination
of Ecotoxicity, Annex to Commission Regulation (EC) No 440/2008 (O.J.
No. L142/456, 2008) Part C, Method 2 “Daphnia sp. Acute Immobilisation
Test” and the OECD Guideline for Testing of Chemicals No. 202, “Daphnia
Acute Immobilisation Test” (2004).
A group of twenty Daphnia, less than 24 hours old, was exposed for 48
hours to Xylanase, PPQ 30189 at a nominal concentration of 100 mg TOS
(Total Organic Solids)/L. The test medium was prepared in Elendt M4
medium by the direct addition of the test substance to the dilution
At the request of the Sponsor, the test concentrations were verified by
analysis of the enzyme concentrations, which was performed at the
Observations of the Daphnia in each control and test vessel were made
after 24 and 48 hours.
No immobilisation or adverse effects on the Daphnia were noted at a
nominal concentration of 100 mg TOS/L (22.2 mg aep/L).
Based on these findings the following values have been estimated:
Xylanase was not toxic towards daphnia magna and hence the 48 h EC50
value for xylanase could not be determined and is above > 100 mg TOS/L
corresponding to 22.2 mg aep/L. The NOEC value was determined to be
>=100 mg TOS/L or 22.2 mg aep/L. The NOEC is >= 100 mg TOS/L equivalent
to 22.2 mg aep/L.
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