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EC number: 232-800-2
CAS number: 9025-57-4
A study was performed to assess the acute toxicity of Xylanase, PPQ
30189 to rainbow trout (Oncorhynchus mykiss) under semi-static
conditions with daily renewal of the media. The active component of the
test substance is an enzyme, which was known to be pH sensitive with
reduced enzymatic activity under alkaline conditions. In order to
maintain the stability of the enzyme the dilution water used in the
study was adjusted to pH 6 before use.
The study was conducted in accordance with EC Methods for Determination
of Ecotoxicity, Annex to Directive 92/69/EEC (O.J. No. L383A, 1992) Part
C, Method 1 “Acute Toxicity For Fish” and the OECD Guideline for Testing
of Chemicals No. 203 “Fish, Acute Toxicity Test” (1992).
A group of ten juvenile fish was exposed to Xylanase, PPQ 30189
dispersed in water at a nominal concentration of 100 mg TOS (Total
Organic Solids)/L; the test medium was prepared by direct addition of
the test substance to water. At the request of the Sponsor, the test
concentrations were verified by analysis of the enzyme concentrations,
which was performed at the Sponsor’s laboratory.
Observations of the fish were made after approximately 2, 4, 24, 48, 72
and 96 hours of exposure. After 96 hours, no mortality or significant
sub-lethal effects on the fish were observed at a nominal concentration
of 100 mg TOS/L (22.2 mg aep/L). Based on these findings the following
values have been estimated: 96-hour LC50 value : >100 mg TOS/L (22.2 mg
aep/L) “No observed effect concentration” : 100 mg TOS/L (22.2 mg aep/L).
Xylanase was not toxic at the tested concentration of 100 mg TOS/L (22.2
mg aep/L) and thus the NOEC was determined to be 100 mg/L (22.2 mg
aep/L).The LC50 value could not be determnined due to the absence of
toxicity of the test compound at the tested concentration.
The acute toxicity of xylanase to
Rainbow Trout (Oncorhynchus mykiss) was determined in a 96 hour
semistatic test with a daily test medium renewal according to the OECD
Guideline No. 203 and in compliance with GLP.
A limit test was performed to
demonstrate that the test substance has no toxic effect on the test
animals up to the concentration of nominal 100 mg test substance/L
corresponding to 22.2 mg active enzyme protein (aep)/L.
The 96h NOEC was determined to be 100
mg/L corresponding to 22.2 mg aep/L (nominal). The 96h LC50 value could
not be determined due to the absence of toxicity of the test substance
but is clearly higher than 100 mg/L (22.2 mg aep/L)(nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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