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EC number: 232-800-2
CAS number: 9025-57-4
The ready biodegradability of Xylanase, PPQ 30189 was assessed in
accordance with EC Directive 440/2008, C.4-D, ‘Determination of Ready
Biodegradability, Manometric respirometry’ and OECD Procedure 301F
‘Ready Biodegradability, Manometric Respirometry Test’, adopted 17 July
The main biodegradability test was preceded by chemical oxygen demand
(COD) analysis as the theoretical oxygen demand of the test substance
could not be calculated.
Xylanase, PPQ 30189 was added to two flasks containing mineral salts
medium inoculated with activated sludge (30 mg solids/L) to give a
nominal test concentration of 50 mgO2/L. Control groups comprised two
cultures containing inoculated mineral salts medium alone. Allylthiourea
(11.6 mg/L) was added to one control culture and to one culture
containing the test substance in order to assess nitrification processes
by the test substance. The test system comprised of an automated system
for oxygen (O2) generation and the cultures were stirred and held in a
thermostatically controlled water bath.
The COD of Xylanase, PPQ 30189 was found to be 0.13 mgO2/mg.
The blank-corrected oxygen demanded by the culture containing the
reference substance had achieved 15.3 mgO2/500 mL or 61% of the ThOD (25
mgO2/500 mL) after 3 days of incubation and 22.2 mgO2/500 mL or 90% by
Day 28. Cumulative levels of oxygen consumption by the controls after 28
days (10.8 and 10.6 mgO2/500 mL, equivalent to 21.6 and 21.2 mgO2/L)
were considered to be acceptable for this assay system.
Mean oxygen consumption in mixtures containing Xylanase, PPQ 30189 was
equivalent to 10% of the theoretical value (25 mgO2/500 mL) after
approximately 1 day, 62% after 3 days and 114% at the end of the test
(Day 28). The degradation of the test substance beyond 100% was related
to the high levels of nitrogen contained in the test substance.
Substances are considered to be readily biodegradable in this type of
test if oxygen consumption is equal to or greater than 60% of the ThOD
of the test mixtures within ten days of the consumption achieving 10%.
Therefore Xylanase, PPQ 30189 was considered to be readily biodegradable
under the conditions of this test.
The enzyme was found to be readily biodegradable according to OECD 301F.
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