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EC number: 201-732-5
CAS number: 87-22-9
Table 1.Skin Reaction Scoring System
Barely perceptible erythema
Scattered, mild erythema (faint pink)
Moderate and diffuse erythema (pale pink )
Intense erythema (deep pink) and oedema
Table 2.1.RESULTS OF CHALLENGE 1 (CONCENTRATION: 10%)
No. of animals
No of positive reactions
Table 2.2 Treated Controls of Challenge 1(Concentration: 10%)
Table 2.3. Untreated Controls of Challenge 1 (Concentration: 10%)
Table 3.1.RESULTS OF CHALLENGE 2 (CONCENTRATION: 10%)
Table 3.2. Untreated Controls of Challenge 2 (Concentration: 10%)
Kligman Guinea pig maximization test was conducted using 10 albino
Dunkin/Hartley guinea pigs for test chemical. Preliminary irritation
tests are carried out in guinea pigs to determine the concentration of
test substances suitable for induction of sensitization and for
sensitization challenge. As a result of the preliminary studies, the
concentration selected for skin sensitization test were 0.5% for
intradermal induction, 50% for topical induction and 10% for challenge.
Induction consisted of intradermal injections followed 1week later by a
48 h occluded patch. Three pairs of intradermal injections were made as
follows: two 0.1 ml injections of 0.5% test chemical in 6% acetone/20%
PEG400/0.01% Tween 80/saline; two 0.1 ml injections of 0.5% test
chemical in 50% FCA; and two 0.1 ml injections of 50% FCA. Seven days
later, an occluded patch with 50% test chemical in acetone was applied
over the shoulder injection sites for 48 h. After a 14-day rest period,
the animals were challenged with 10% test chemical in acetone applied
for 24 h under occlusion using an 11 mm aluminum patch test cup.
Reactions were read 24 and 48 h after patch removal. Second challenge
was conducted 1 week after on the opposite flank exactly as per the
primary challenge. Further challenges and cross challenges may be made
on alternate flanks at weekly or greater intervals as required with
controls included. Sensitization reactions were observed after both
challenges. Cross-challenge applications with 10% benzyl salicylate and
10% phenyl salicylate were then made at weekly intervals.
Cross-reactions were observed after each challenge. Approximately 3 out
of 10 guinea pigs showed positive skin sensitization reaction after 2
challenges. Hence the chemical was considered to as weak sensitizer on
albino Dunkin/Hartley guinea pigs.
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