Phenethyl salicylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Toxicity to micro-organisms study was conducted for 24 hrs using five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp., respectively.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

yes

Remarks:

ethyl alcohol or DMSO was used as a vehicle.

Details on test solutions:

- Sampling method: Test chemical solutions were prepared in either ethyl alcohol or DMSO depending on test chemical solubility.

Test organisms (species):

other: Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter sp.

Details on inoculum:

- Laboratory culture: Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var. (SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66.

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Test temperature:

37°C

Details on test conditions:

TEST SYSTEM
- Test vessel: Culture dish was used as a test vessel.
- Material, size, headspace, fill volume: Culture dish of 35 X 10 mm dimension was used.
 
TEST MEDIUM / WATER PARAMETERS: Muller Hinton agar was used as a test medium.
 
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Minimum inhibitory concentration (MIC) was determined as the concentration where no growth of the test organism was observed after 24 hrs.
 

Reference substance (positive control):

no

Key result

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

> 2 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.

Executive summary:

Toxicity to micro-organisms study was conducted using five different microorganisms.The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university.Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10E6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.

Description of key information

Toxicity to micro-organisms study was conducted using five different microorganisms. The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10e6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

2 000 mg/L

Additional information

Toxicity to micro-organisms study was conducted using five different microorganisms. The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10e6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.