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EC number: 201-732-5
CAS number: 87-22-9
Biodegradation in water:
The ready biodegradability of test
chemical was determined in accordance with the OECD Guideline 301 F
(Ready Biodegradability: Manometric Respirometry Test) (Experimental
study report, 2000). Fresh activated sludge from a biological wastewater
treatment plant treating predominantly domestic sewage was used. The
sludge is collected in the morning, washed three times in the mineral
medium (by centrifuging at 1000 g for 10 minutes, discarding the
supernant and resuspending in mineral medium) and kept aerobic until
being used on the same day. Initial test substance concentration used in
the study was 100 mg/l. Mineral medium was prepared by mixing 50 ml of
solution A and 2000 ml deionised water, adding 5 ml of each of the
solutions B, C and D and making up to 5 litres with deionised water. The
pH is measured and if necessary, adjusted to 7.4 +/- 0.2 with phosphoric
acid or potassium hydroxide. The water used during this study is
deionised water containing less than 10 mg/l dissolved organic carbon.
Suspended solids concentration used in the study was 3.289 g/l. To
obtain a concentration of inoculum of 30 mg/l (dry weight) in a 250 ml
flask, 2.28 ml of sludge is needed (inoculum). Sodium benzoate (100
mg/l) (purity min. 99.0%) was used as a reference substance. Degradation
of sodium benzoate exceeded 40% after 7 days and 65% after 14 days, the
activity of the inoculum was thus verified, and the test is considered
as valid. Test chemical undergoes 93% biodegradation after 28 days in
the test conditions. Biodegradation starts on day 3 and reached 80% at
the end of the 10-day window (day 3 to 13). The curves obtained with the
reference substance alone and with test chemical plus reference
substance show no toxic effect of test chemical to the micro-organisms
at the test concentration (100 mg/l). Thus, test chemical is considered
as readily biodegradable.
Biodegradation in water and
sediment: simulation test
accordance with column 2 of Annex IX of the REACH regulation, testing
for this end pointis
scientifically not necessary and does not need to be conducted since the
readily biodegradable in water.
Biodegradation in soil
In accordance with column 2 of Annex
IX of the REACH regulation, testing for this end point is scientifically
not necessary and does not need to be conducted since the test chemical
is readily biodegradable in water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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