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EC number: 214-881-6
CAS number: 1205-17-0
Table 1: Summary of Experiment B1
± S9 Mix
Mean Revertants / plate
Base-pair Substitution Type
Mean no. colonies/plate
MMS = Methyl methanesulfonate
9AA = 9-aminoacridine
2AA = 2-aminoanthracene
SA = Sodium azide
2NF = 2-Nitrofluorene
Table 2: Summary of Experiment B2
Mean number of colonies/plate in strain TA1537
The mutagenic activity of the test material was evaluated in a bacterial
reverse mutation assay conducted using a protocol written to comply with
the standardised guideline OECD 471 under GLP conditions.
S. typhimurium tester strains TA98, TA100, TA1535 and TA1537 and E.coli
tester strain WP2uvrA were exposed to the test material in the presence
and absence of Aroclor-induced rat liver S9 using the plate
incorporation method. Following a preliminary toxicity assay, the
mutagenicity assay (Experiment B1) was used to evaluate the mutagenic
potential of the test material at concentrations of 0, 25, 75, 200, 600,
1800 and 5000 µg/plate in DMSO. Appropriate vehicle and positive
controls for each tester strain were evaluated concurrently. All dose
levels of test material, vehicle controls and positive controls were
plated in triplicate.
No precipitate was observed but toxicity was generally observed at 5000
µg per plate. In Experiment B1, no positive responses were observed with
any of the tester strains in the presence and absence of S9 activation.
However, a non-dose responsive increase was observed with tester strain
TA 1537 (1.7-fold, maximum increase) in the presence of S9 activation.
This strain/activation condition was retested in Experiment B2 to
clarify the response observed. In Experiment B2, no positive response
was observed with tester strain TA1537 in the presence of S9 activation.
All criteria for a valid study were met. The results indicate that under
the conditions of this study the test material did not cause a positive
response with any of the tester strains in the presence and absence of
Aroclor-induced rat liver S9. The study was concluded to be negative
without conducting a full independent repeat assay because no unique
metabolism requirements were known about the test material and because
the equivocal response was retested to resolve the nature of the
Under the conditions of this study, the test material was concluded to
be negative with regard to genotoxicity in the Bacterial Gene Mutation
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