Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3-chloro-2-hydroxypropyl)dimethyloctadecylammonium chloride
EC Number:
221-083-1
EC Name:
(3-chloro-2-hydroxypropyl)dimethyloctadecylammonium chloride
Cas Number:
3001-63-6
Molecular formula:
C23H49ClNO.Cl
IUPAC Name:
(3-chloro-2-hydroxypropyl)dimethyloctadecylazanium chloride
Constituent 2
Chemical structure
Reference substance name:
Propane-1,2-diol
EC Number:
200-338-0
EC Name:
Propane-1,2-diol
Cas Number:
57-55-6
Molecular formula:
C3H8O2
IUPAC Name:
propylene glycol
Test material form:
liquid
Details on test material:
Aqueous solution

Test solutions

Vehicle:
yes
Details on test solutions:
100 mg of QUAB 426 was accurately weighed out and dissolved in 1000 ml of dilution water. From this solution 1.0, 1.8, 3.2, 5.6 and 10 ml were taken
and diluted to one Litre with dilution water. These solutions, containing 0.1, 0.18, 0.32, 0.56 and 1.0 mg/l were used as test solutions.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
The organism used was the fresh-water fish species Brachydanio rerio cultured in the Laboratory. The test animals were 1.7 +/- 0.2 cm long and weighed 0.04 +/- 0.01 g.

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
Expressed as CaCo3, approx 210 mg/l
Test temperature:
Test solutions were kept at 24°C +/- 1°C
pH:
The pH of the test and control solutions varied between 8.0 and 8.3.
Dissolved oxygen:
The oxygen concentrations of the test and control solutions were greater than 7.3 mg.
Details on test conditions:
the five concentrations of the test substance were tested. The test was performed in 1000 ml all-glass beakers , each containing 1000 ml of test solution. Two beakers containing 5 fishes each were used for each test or control solution. The test lasted 96 h. The test and control solutions were renewed daily. The fishes were not fed. At the beginning and at the end of the test and at each renewal time the pH and the oxygen concentration of the test and control solutions were measured. The test solutions were kept at 24 +/- °C and slightly aerated. The dead animals were counted and removed at least after 24 h, 48 h, 72 h and 96 h. At the same times the condition of the survivors was compared with that of the control animals in the blanks.
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 0.34 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
ca. 0.18 mg/L
Conc. based on:
test mat.
Basis for effect:
behaviour

Applicant's summary and conclusion

Conclusions:
The LC50 (96h) of the test solution was found to be 0.34 mg/l. Based on a concentration of the substance of 40 %, the LC50 of 3-Chloro-2-hydroxypropyldimethyloctadecylammoniumchloride woudl be 0.85 mg/l.