Benzene, di-C10-14-alkyl derivs., sulfonated, sodium salts

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

not specified

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzene, di-C1 0-14-alkyl derivs., sulfonated, sodium salts

IUPAC Name:

Benzene, di-C1 0-14-alkyl derivs., sulfonated, sodium salts

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Benzene, di-C1 0-14-alkyl derivs., sulfonated, sodium salts
- ZS name: ALKYLBENZOLNACHLAUF, SULFONIERT, NA-SALZ
- ZS number: 1344
- Batch: SEALS 2011-104-5-8

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Conditions: animals were housed individually in labeled Makrolon cages
Temperature: 20 - 23ºC and relative humidity: 30-45%
12 hours artificial fluorescent light and 12 hours darkness per day
Acclimatization period: 14 days before start of treatment under laboratory conditions
Diet: free access to pelleted rodent diet Altromin 1324
Water: free access to tap water

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Mortality, body weight, behavior, toxicity symptoms and anomalies were recorded during the 14 days after the exposure

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality observed

Clinical signs:

No clinical signs observed

Body weight:

No significant changes observed

Gross pathology:

No anomalies were observed following gross pathology examination

Applicant's summary and conclusion

Conclusions:

Under the study conditions, the rat LD50 of the test substance was determined to be >2000 mg/kg bw.

Executive summary:

A study was conducted to determine the acute oral toxicity of the test substance according to OECD Guideline 423. Male and female Wistar rats were exposed by oral gavage at a concentration of 2000 mg/kg bw. Treatment was followed by a 14 d observation period. No mortality, significant body weight changes, clinical signs or anomalies following gross pathology examination were observed. Under the study conditions, the LD50 of the test substance to rat was determined to be >2000 mg/kg bw (Müller, 2001).