Tetraoctylammonium bromide

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate was predicted Tetraoctylammonium bromide (CAS: 14866-33-2). Effect concentration i.e EC50 value was estimated to be 148.66 mg/l for Daphnia magna for 48 hrs duration. It was concluded that the Tetraoctylammonium bromide (CAS: 14866-33-2) was likely to be not toxic to aquatic invertebrate, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae  predicted for Tetraoctylammonium bromide (CAS: 14866-33-2). Effect concentration i.e EC50 value estimated to be 116.59 mg/l for Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72 hrs duration. It can be concluded that the Tetraoctylammonium bromide (CAS: 14866-33-2) likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to aquatic invertebrates

Following studies includes predicted data and experimental data of target chemical and structurally similar read across to conclude the

Tetraoctylammonium bromide (CAS: 14866-33-2) toxicity towards the aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate was predicted Tetraoctylammonium bromide (CAS: 14866-33-2). Effect concentration i.e EC50 value was estimated to be 148.66 mg/l for Daphnia magna for 48 hrs duration. It was concluded that the Tetraoctylammonium bromide (CAS: 14866-33-2) was likely to be not toxic to aquatic invertebrate, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

The predicted data is supported by the experimental data of structurally similar read across Methyloxirane (CAS: 75-56-9) from European Union Risk Assessment Report 2002, suggests that the Short-term toxicity to aquatic invertebrates test carried out according to US–EPA–660/3–75–009 test guidelines to study the effects of Methyloxirane on aquatic environment. test vessels were completely filled and sealed (closedsystem). Test condition were pH 8,0 – 8,1; 18 – 22 degree C; dissolved oxygen: 9.0 – 9.2 mg/l;Measured endpoint was immobilization.

The Effective concentration EC50 50% of Daphnia magna (Crustacea) at 48 hour(s) exposure period is 350 mg/l. It can be concluded that the Methyloxirane is not toxic to the aquatic environment.

Similar experimental study for another read across Docosane(CAS: 629-97-0) from Bulletin of Environmental Contamination and Toxicology 1980, indicats that the Short-term toxicity to aquatic invertebrates test as carried out according to the Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (U.S. EPA 1975) to study the effects of Docosane on aquatic invertebrate. Daphnia magna (<24 hours old) used in these toxicity tests were from laboratory stocks cultured at EG&G, Bionomics. Water used to culture the organisms used in the initial tests was deionized reconstituted well water having a total hardness of 72 + 6 mg/L as CaCO3 and a pH of 7.0 + 0.2. Subsequently culture water was reconstituted according to U.S. EPA (1975) s a total Hardness of 173 _+ 13 mg/L as CaCO 3 and a pH of 8.0 - 0.2, to improve conditions for test organisms. If no mortality was observed in water flea populations exposed to a nominal concentration of approximately 500 mg/L, no additional concentrations were tested and the LC50 was estimated to be greater than the highest nominal concentration tested (>530 mg/L).

The Lethal concentration LD50 to 50% ofDaphnia magna at 48 h is530 mg/L. Increasing trend of mortality was observed as effect measurement. It can be concluded form the mortality value that the Docosane is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 148.66 mg/l to530 mg/lgive the conclusion that test substanceTetraoctylammonium bromide (CAS: 14866-33-2)is likely to be non-toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies includes predicted data and experimental data of target chemical and structurally similar read across to conclude the

Tetraoctylammonium bromide (CAS: 14866-33-2) toxicity towards the aquatic algae by applying weight of evidence aprpoach is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae  predicted for Tetraoctylammonium bromide (CAS: 14866-33-2). Effect concentration i.e EC50 value estimated to be 116.59 mg/l for Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72 hrs duration. It can be concluded that the Tetraoctylammonium bromide (CAS: 14866-33-2) likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The predicted data is supported by the experimental data of structurally similar read across Methyloxirane (CAS: 75-56-9) from European Union Risk Assessment Report 2002, suggests that the Toxicity to aquatic algae and cyanobacteria test was carried out according to US–EPA–660/9–78–018 test guidelines to study the effects of Methyloxirane on aquatic environment. The effective concentration EC50 to 50% of Selenastrum capricornutum at 96 hour(s) is 240 mg/l. It can be concluded that the Methyloxirane is not toxic to the aquatic environment.

Similar experimental study for another read across Docosane(CAS: 629-97-0) from European Union Risk Assessment Report 1978, indic.ates that the

Toxicity to aquatic algae and cyanobacteria test s carried out to study the effects of Docosane in aquatic invertebrate.

The Effective concentration EC50 to 50% ofSkeletonema costatum at 96 h is 500 mg/l. It can be concluded that the Docosane is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 116.59 mg/l to500 mg/lgive the conclusion that test substanceTetraoctylammonium bromide (CAS: 14866-33-2)is likely to be non-toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.