Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-334-4 | CAS number: 94-46-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Special Microbiological Systems. 11. Observations On Chemical Mutagenesis In Microorganisms
- Author:
- Waclaw Szybalski
- Year:
- 1 958
- Bibliographic source:
- Annals of the NY Academy of Science, Vol: 76 2c PP 475-489, 1958
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Paper-disk method was performed to determine the mutagenic nature of isoamyl benzoate using E. coli Sd-4-73 by checking its reversion from streptomycin dependence to independence
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Isopentyl benzoate
- EC Number:
- 202-334-4
- EC Name:
- Isopentyl benzoate
- Cas Number:
- 94-46-2
- Molecular formula:
- C12H16O2
- IUPAC Name:
- isopentyl benzoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: Isoamyl benzoate
- IUPAC name: 3-methylbutyl benzoate
- Molecular formula: C12H16O2
- Molecular weight: 192.256 g/mol
- Substance type: Organic
- Physical state: Liquid
- Purity: No data available
- Impurities (identity and concentrations): No data available
Method
- Target gene:
- Streptomycin specific gene
Species / strain
- Species / strain / cell type:
- E. coli, other: Sd-4-73
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- not specified
- Metabolic activation system:
- No data
- Test concentrations with justification for top dose:
- 0.01 to 0.025 ml or crystals of chemical
- Vehicle / solvent:
- No data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: As impregnation on paper disk.
DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): 30 µg of sulfocidin and unidentified fungus was added.
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: ): No data available
NUMBER OF CELLS EVALUATED: 109 cells/ml
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other:
OTHER: - Rationale for test conditions:
- No data
- Evaluation criteria:
- Increase in the frequency of reversion from streptomycin dependence to independence in strain Sd-4-73 of Escherichia coli.
- Statistics:
- No data
Results and discussion
Test results
- Species / strain:
- E. coli, other: Sd-4-73
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No data available
Applicant's summary and conclusion
- Conclusions:
- Isoamyl benzoate did not induce mutation from streptomycin dependence to independence in Escherichia coli (strain Sd-4-73) and hence the chemical is not likely to classify as a gene mutant in vitro.
- Executive summary:
Gene mutation toxicity study was performed to determine the mutagenic nature of Isoamyl benzoate. Genetic toxicity test was performed on strain of Escherichia coli (strain Sd-4-73) by paper disk method. Paper-disk method was performed to check for the ability of E. coli Sd-4-73 to show reversion from streptomycin dependence to independence. Overnight culture of strain Sd-4-73 grown at 36°C in aerated nutrient broth containing 20 µg/ml of streptomycin was used as a inoculum. The culture was centrifuged and washed at least twice with saline or distilled water to remove the extraneous streptomycin, and was resuspended in saline to a concentration of approximately 109 cells/ml. 0.1-ml. aliquot of this suspension was mixed with 2.5 ml of molten soft nutrient agar (0.7 per cent agar) and poured over a base of 20 ml of 1.5 per cent nutrient agar. After the soft layer was solidified, the mutagen was applied in form of small drops or crystal. Additional plates were prepared with small inocula (one fifth and one twenty-fifth of the original) so as not to miss the optimum cell density; the number of cells per plate was rather critical, the yield of mutant colonies being reduced either by crowding or by insufficient population size. After the soft agar layer had set, the mutagen, in the form of a microdrop of solution (0.01 to 0.025 ml.) or a small crystal, was applied to a small filter-paper disk resting on the agar. To determine whether the substance was inhibitory for the assay organism at the concentration employed, the procedure was repeated on a nutrient agar plate containing 100 µg/ml of streptomycin and seeded with approximately 107 bacteria. Mutagenicity was manifested as a zone of streptomycin-independent mutant colonies around a filter-paper disk saturated with the mutagenic agent and resting on the surface of streptomycin-free nutrient agar seeded heavily with a streptomycin-dependent parental population. Isoamyl benzoate did not induce mutation from streptomycin dependence to independence in Escherichia coli (strain Sd-4-73) and hence the chemical is not likely to classify as a gene mutant in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
