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EC number: 257-055-0
CAS number: 51202-86-9
The test compound was suspended in DMSO and a stock solution of 50 mg/ml
was prepared for the highest concentration, which provided a final
concentration of 5000 µg/plate. Further dilutions of 2500, 500, 100, 20
and 4 µg/plate were used in the first experiment.
Visible precipitation of the test compound on the plates was observed at
500 µg/plate and above. Because of heavy precipitation of the test
compound the bacterial lawn could only be evaluated at the dose level of
2500 µg/plate and lower doses.
The test compound proved to be toxic to most of the bacterial strains at
concentrations of 500 µg/plate and above. Thinning of bacterial lawns
and in most cases also a reduction in the number of colonies were
observed at these doses.
Because of heavy precipitation in the first experiment dose ranges from
0.8 to 2500 µg/plate were chosen for the second experiment. The toxic
effects were in most cases reproduced with the Salmonella typhimurium
strains in this experiment.
A toxicity test using histidine-enriched agar plates and a dilution of
the tester strain TA 100 (designated TA 100 D) was performed in parallel
with the second experiment. Toxicity was found at concentrations of 500
µg/plate and above in the absence of metabolic activation. In the
presence of metabolic activation the test compound proved to be not
toxic to the bacterial strain.
In all independent mutation tests the test item was tested for
mutagenicity with the same concentrations as described above. The number
of colonies per plate with each strain as well as mean values of 3
plates are given. The test compound did not cause a significant increase
in the number of revertant colonies with any of the tester strains
either in the absence or in the presence of S9-mix in either mutation
test. No dose-dependent effect was obtained.
All positive controls produced significant increases in the number of
revertant colonies. Thus, the sensitivity of the assay and the efficacy
of the exogenous metabolic activation system were demonstrated.
The results lead to the conclusion that the test item is not mutagenic
in these bacterial test systems either in the absence or in the presence
of an exogenous metabolizing system.
In a bacterial reverse mutataion assay (Ames test) employing four
strains of Salmonella typhimurium with and without metabolic activation
no relevant increase of revertant colonies was recorded.
The test material is considered not mutagenic.
This finding was also confirmed in an in vivo mouse micronucleus assay.
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