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EC number: 257-055-0
CAS number: 51202-86-9
see table below
In order to study a possible contact allergenic potential of the test
item, three groups each of four female mice were treated daily with the
test item at concentrations of 6.25, 12.5, and 25% (w/v) in
acetone:olive oil (4+1) by topical application to the dorsum of each ear
lobe (left and right) for three consecutive days.
A control group of four mice was treated with the vehicle (acetone:olive
oil (4+1)) only.
Five days after the first topical application the mice were injected
intravenously into a tail vein with radio-labelled thymidine (3H-methyl
thymidine). Approximately five hours after intravenous injection, the
mice were sacrificed, the draining auricular lymph nodes excised and
pooled per group. Single cell suspensions of lymph node cells were
prepared from pooled lymph nodes, which were subsequently washed and
incubated with trichloroacetic acid overnight. The proliferative
capacity of pooled lymph node cells was determined by the incorporation
of 3H-methyl thymidine measured in a ß-scintillation counter.
All treated animals survived the scheduled study period and no signs of
toxicity were observed.
A test item is regarded as a sensitiser in the LLNA if the exposure to
one or more test concentration resulted in 3-fold or greater increase in
incorporation of 3HTdR compared with concurrent controls, as indicated
by the Stimulation Index (S.I.). The estimated concentration of test
item required to produce a S.I. of 3 is referred to as the EC3 value.
In this study Stimulation Indices (S.I.) of 2.1, 1.8, and 0.7 were
determined with the test item at concentrations of 6.25, 12.5, and 25%
in acetone:olive oil (4+1), respectively. The EC3 value could not be
calculated, since none of the tested concentrations induced an S.I.
greater than 3.
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