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EC number: 257-055-0
CAS number: 51202-86-9
Methods of evaluation
The effects of the saturated solution were deduced directlyfrom
the observed immobilization rates.
Alleffect levels (EL10
/ 50 / 100) given were based on the nominal test item
EC-values and statistical
analyses for reference item
An EC50-value was calculated for the reference item by
sigmoidal dose-response regression.The
respective 95% confidence limits were calculated from the standard error
and the t-distribution. All calculations were carried out from the
best-fit values with the software GraphPad Prism5.
All data were computer-processed and rounded for presentation.
Consequently, minor variations may occur from the original figures if
manual calculations based on the original figures are made subsequently.
Calculations were made using the following software:
- GraphPad Prism5,GraphPad
In an acute immobilization test with Daphnia magna (STRAUS),
the effects of a saturated solution with a nominal loading of 1.00 mg/L
of the test item were determined at the test facility according to OECD
202 (2004) from2016‑09‑11 to 2016-09-15, with the definitive exposure
phase from 2016-09-13 to 2016-09-15.
The limit test was conducted under static conditions over a period
of 48 hours.The saturated solution was colorless and visually clear
during the exposure period. Twenty daphnids, divided into 4 replicates
with 5 daphnids each, were exposed to the saturated solution and the
No analytical monitoring was performed during the test period in
agreement with the sponsor, since no suitable method for the
determination of the test item could be established. The test item is
insoluble in water and organic solvents which are compatible for liquid
chromatography.Exposures are expressed in terms of the nominal
concentration of the test itemPolysynthren-GelbGG.
The validity criteria of the test guideline
EL10 / 50 / 100-Values
on the nominal loading of the test item)
Nominal test item loading
EL10 / 50 / 100
In the saturated solution with a
nominal loading of 1.00 mg/L of the test item no effects
onDaphnia magnawere observed.
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