Fatty acids, C10-20-neo-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted accorrding to accepted OECD protocols and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C10-20-neo-

EC Number:

285-549-6

EC Name:

Fatty acids, C10-20-neo-

Cas Number:

85116-96-7

Molecular formula:

C10H20O2 ... C20H40O2

IUPAC Name:

C10-20-neo-Fatty acids

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

water accommodated fraction: loading rated of 0.3 - 30 mg/L. Stirred for 69-73 hours

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Healthy O. mykiss fingerlings were obtained from Zeals Trout Farm, Zeals, Wiltshire, and acclimated to the test conditions for more than nine days before use. A sample of seven of the fish used in the test had a mean length of 4.6 cm and a mean weight of 0.76 g.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

276-292mg/l as CaCO3

Test temperature:

15.4-16.3 C

pH:

7.1-7.6

Dissolved oxygen:

8.6-9.1 mg/l

Details on test conditions:

A sealed 96 h semi-static toxicity test was carried out with daily renewal of the test media. The test vessels were glass aspirators which were completely filled (11 liters) with WAF of VERSATIC 1019. Seven O. mykiss were placed in each vessel. The vessels were then sealed with glass plated ensuring that all air was excluded. The fish were not fed during the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

10 - 30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Conclusions:

The 96 h LL50 of VERSATIC 1019 as determined by a OECD 203 Acute fish toxicity test is between 3 and 10 mg/l.

Executive summary:

The acute toxicity to O. mykiss of water accommodated fractions of VERSATIC 1019 was determined in a 96 h semi-static toxicity test with daily renewal of the test media. The test was conducted in seled test vessels. The 24, 48, 72 and h LL50 values (loading rates producing WAF resulting in mortality of 50% of the fish after 24, 48, 72 and 96 h exposure) were within the ranges 10 -30, 3 -10, 3 -10 and 3 -10 mg/l respectively.