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EC number: -
CAS number: -
Verification of Test Concentrations
Analysis of the test preparations at
0, 24, 72 and 96 hours showed measured test concentrations to be near
nominal and so it was considered justifiable to estimate the LC50 values
in terms of the nominal test concentrations only.
The test was considered to be valid
given that none of the control fish died or showed signs of stress
during the test and that the oxygen concentration at the end of the test
was ≥60% of ASV (6.1 mg O2/L) in the control and test
Inspection of the mortality data gave
the following results:
Water Quality Criteria
Temperature was maintained at
approximately 14 °C throughout the test, while there were no treatment
related differences for oxygen concentration or pH.
Observations on Test Item Solubility
The test solutions were observed to be
an extremely dark blue solution throughout the test.
Cumulative Mortality Data in the
Cumulative Mortality (Initial Population = 7)
Water Quality Measurements
0 Hours (Fresh Media)
24 Hours (Old Media)
24 Hours (Fresh Media)
48 Hours (Old Media)
48 Hours (Fresh Media)
72 Hours (Old Media)
72 Hours (Fresh Media)
96 Hours (Old Media)
A study according to OECD guideline
203 and EU Method C.1, was performed to assess the acute toxicity of the
test item to rainbow trout (Oncorhynchus mykiss). The test was
conducted at a single concentration of 100 mg/L to
ensure that toxicity was not observed at this concentration. Seven
fish were exposed to an aqueous solution of the test item, at a single
concentration of 100 mg/L for a period of 96 hours at a temperature of
approximately 14 °C under semi-static test conditions. The number of
mortalities and any sub-lethal effects of exposure in each test and
control vessel were determined 3 and 6 hours after the start of exposure
and then daily throughout the test until termination after 96 hours.
Analysis of the test preparations at 0, 24, 72 and 96 hours showed
measured test concentrations to be near nominal and so the results are
based on nominal test concentrations only. No mortality was observed
throughout the duration of exposure. Hence, 96 -hour LC50 of the test
item to the freshwater rainbow trout (Oncorhynchus mykiss) was
determined to be >100 mg/L. The No Observed Effect Concentration was 100
The 96-Hour LC50 of the test item to freshwater rainbow trout was >100 mg/L.
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