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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from authoritative database

Data source

Reference
Reference Type:
other: authoritative database
Title:
Eye irritation study on rabbits
Author:
US National Library of Medicine (NLM)
Year:
2015
Bibliographic source:
Canadian Centre for Occupational Health and Chemistry

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as below
Principles of method if other than guideline:
Range finding study was conducted to evaluate the eye irritation potential of the test compound Thiosemicarbazide
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiosemicarbazide
EC Number:
201-184-7
EC Name:
Thiosemicarbazide
Cas Number:
79-19-6
Molecular formula:
CH5N3S
IUPAC Name:
hydrazinecarbothioamide
Details on test material:
- Name of test material (as cited in study report): Thiosemicarbazide
- Molecular formula (if other than submission substance): CH5N3S
- Molecular weight (if other than submission substance): 91.1375 g/mol
- Substance type: Organic
- Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
Sex: Male

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
0.1 g
Duration of treatment / exposure:
Duration of treatment: 7 days
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
6
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): ): 20- 30 sec after the exposure in 3/6 male rabbits
- Time after start of exposure:

SCORING SYSTEM: Ocular irritation of cornea, iris, conjunctivae

TOOL USED TO ASSESS SCORE: No data

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 7 days
Reversibility:
not specified
Remarks on result:
other: No ocular irritation (Draize) of cornea, iris, or conjunctivae was observed.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Thiosemicarbazide is not irritating to the eyes of New Zealand White rabbits.
Executive summary:

Eye irritation study was performed male New Zealand White rabbits to evaluate the irritation potential of the test compound Thiosemicarbazide.

 

The test chemical was instilled as a single dose of 0.1g in the eyes of rabbits. Half (3/6) of the eyes were cleansed at 20-30 seconds after the exposure.

 

No ocular irritation (Draize) of cornea, iris, or conjunctivae was reported in any animal throughout 7-day post-instillation observation.

 

Thiosemicarbazide is not irritating to the eyes of New Zealand White rabbits.

 

In accordance with the CLP classification, the test material Thiosemicarbazide does not classify as an eye irritant.