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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11-13 September 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted in GLP compliance and in accordance with internationally acknowledged guidelines for testing of chemicals.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Chlorobenzoylacetonitril
IUPAC Name:
Chlorobenzoylacetonitril
Test material form:
other: solid
Details on test material:
- Name of test material (as cited in study report): Chlorobenzoylacetonitril
- Physical state: solid
- Analytical purity: > 99%
- Purity test date: 22 Dec 1999
- Lot/batch No.: 46
- Storage condition of test material: stored at 4°C in original container

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: daphnia magna
- Strain: straus, clone 5
- Source: Umweltbundesamt, Berlin, Germany
- Age at study initiation: less than 23.5 hrs


ACCLIMATION
- Acclimation period: 23.5 hrs
- Acclimation conditions (same as test or not): similar conditions


Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
2.5 mmol/L as CaCO3
Test temperature:
21°C
pH:
7.7-8
Dissolved oxygen:
>= 8.3 mg/L
Nominal and measured concentrations:
4.6, 10, 21, 42 and 100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: 100mL glass beakers
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deoinized water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:no
- Photoperiod: 16h light:8h dark
- Light intensity: 370 Lux


TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 4.6, 10, 12 and 42 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.4 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
21 mg/L
Nominal / measured:
nominal

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h NOEC was determined to be 46 mg/L. The 48h EC 50 was determined to be 9.4 mg/L.