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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted in GLP compliance and in accordance with several internationally acknowledged guidelines for the testing of chemicals.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Chlorobenzoylacetonitril
IUPAC Name:
Chlorobenzoylacetonitril
Test material form:
other: solid
Details on test material:
- Name of test material (as cited in study report): Chlorobenzoylacetonitril
- Physical state: solid
- Analytical purity: > 99%
- Purity test date: 22 Dec 1999
- Lot/batch No.: 46
- Storage condition of test material: stored at 4°C in original container

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from a domestic sewage treatment plant, Groß-Zimmern, Germany
- Preparation of inoculum for exposure: Washing with tap water, centrifugation, decantation of supernatant (twice). Drying of sludge susension, weighing, mixing with test water, aeration until use.
- Concentration of sludge: 1.5g dry material/L.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
25.1 other: mg in 244mL
Based on:
test mat.
Initial conc.:
25.6 other: mg in 244 mL
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: analytical grade salts suspended in deionized water
- Test temperature: 22°C
- pH: 7.6 at test start
- pH adjusted: no
- Continuous darkness: yes
- suspended solids: 30mg/L


TEST SYSTEM
- Culturing apparatus: 500mL manometric flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration of sludge prior to use, headspace in flasks filled with air.
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance: flasks were closed


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes, aniline
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
other: ThODNH4
Value:
> 9 - < 12
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Aniline passed the ready biodegradability level of 60% within 5 days. The study is therefore considered valid.

Any other information on results incl. tables

The abiotic control did not induce any oxygen consumption.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
The test substance was determined to be not readily biodegradable.