Tin

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation, other

Remarks:

other: Various - see attached background information

Type of information:

other: Review

Adequacy of study:

supporting study

Study period:

not reported.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

An extensive expert report written by a leading expert in the field of sensitisation assessing the available data in the public domain concerning the potential of metallic tin to act as a sensitiser.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2008

Materials and methods

Principles of method if other than guideline:

A variety of investigational techniques and approaches were used to determine potential sensitising capabilities of metallic tin. These are outlined in the attached background information and summarised in the overall review .

GLP compliance:

no

Type of study:

other: Various - see attached background information

Test material

Test material information

Constituent 1

Reference substance name:

Tin

EC Number:

231-141-8

EC Name:

Tin

Cas Number:

7440-31-5

Molecular formula:

Sn

IUPAC Name:

tin

Constituent 2

Reference substance name:

Tin chloride

EC Number:

215-689-5

EC Name:

Tin chloride

Cas Number:

1344-13-4

IUPAC Name:

tin(4+) tetrachloride

In vivo test system

Test animals

Species:

other: human, guinea pig, rat and mouse data reviewed

Strain:

other: Sensitisation discussed in a variety of species and strains

Sex:

male/female

Study design: in vivo (non-LLNA)

Induction

Route:

other: Various routes of challenge, often not determinable in the human subjects discussed in the various available studies.

Vehicle:

other:

Remarks:

various methods of administration employed in the reviewed studies.

Concentration / amount:

Variable depending of the route of administration

Challenge

Route:

other: various routes of challenge

Vehicle:

other: various methods of administration employed in the reviewed studies.

Concentration / amount:

Variable depending of the route of administration

No. of animals per dose:

There was a large variety in the study size depending on the type of study reviewed in the expert report

Details on study design:

Review of clinical experience, historical data for animal studies

Results and discussion

Any other information on results incl. tables

Based on the available data presented in literature, and studies and testimonies from interviews with dermatologists a strong argument was presented that tin was not a skin sensitiser. Furthermore despite widespread historical of use tin and being freely available to the public, there is no evidence of skin sensitisation in recorded history.

Applicant's summary and conclusion

Interpretation of results:

other: Not classified in accordance with EU criteria according to expert judgement

Conclusions:

The review of available information relating to sensitisation and human contact dermatitis, discussed withtin the expert review, concluded that no classification of metallic tin as a sensitiser is warranted.

Executive summary:

An extensive expert report is available written by a leading expert in the field of sensitisation assessing the available data in the public domain concerning the potential of metallic tin to act as a sensitiser.

Firstly, a salutary reminder: the tin can was invented almost 200 years ago, its success arising in no small part from the resistance of tin to corrosion, dissolution, reaction etc.  From first principles, these properties suggest metallic tin is not likely to represent a significant skin sensitisation hazard.  Indeed, to act as a skin sensitiser, a substance must possess two key properties, the ability to penetrate to a sufficient extent into the viable epidermis (recently reviewed in Basketter et al., 2007) and, once there, to bind firmly with skin proteins (recently reviewed in Divkovic et al., 2005).  It is considered that metallic tin is not readily able to achieve either of these.  Nevertheless, it must be remembered that skin sensitisation can be driven by relatively modest amounts of a substance, so the other data must be considered.

Concerning chemistry, structure activity relationships and so forth, then this is always a problematic territory.  However, tin is clearly not a highly reactive metal and does not reside in the periodic table in close association with known metal skin sensitisers, e.g. Ni, Cr, Co, Au, and it is judged that this therefore should give some degree of confidence that it is unlikely to behave as a skin sensitiser.  Clearly, an expert in the chemistry of metals and their interaction with organic materials such as proteins may be able to clarify this matter further. As far as the review report can ascertain, there is no animal skin sensitisation study of metallic tin.  This is not surprising; many metals have been tested as one or other of their salts, quite often the chloride, as this is the salt normally produced on the skin by interaction with (corrosive) sweat.  The resistance of tin to corrosion means that generation of tin chloride on the skin is considered to be less likely.  It is therefore relevant to note that neither of the tin chloride salts has been evaluated in predictive skin sensitisation assays, and only the divalent salt having been used in diagnostic patch testing (TNO Report, 2007). 

Next, what does the clinical data on tin convey concerning skin sensitisation hazard?  There is initial observational data which is suggestive of some potential, but this has not translated into any significant further evidence some two decades later, either published or in the awareness of leading figures of as yet unpublished information.  Does this represent a quantity of human evidence sufficient to trigger classification according to regulatory criteria?  It is considered that it does not.  The reports represent no more than isolated reports, very largely from a single clinic.  If all of these cases actually were true contact allergy (their clinical relevance was not established, so to refer to them as allergic contact dermatitis is incorrect), then they are not greater in number than the isolated cases of petrolatum allergy that have been reported (Schnuch et al., 2006).  There is of course no question that petrolatum could ever be regarded as anything other than a non-sensitising substance. 

To finish, it is noted that the REACH Regulation, as others before it, requires (a sufficient body of) human evidence of allergic contact dermatitis to trigger classification.  For metallic tin, not only is it no more frequent a contact allergen than petrolatum, in contrast to the latter, it has never been shown to have any relevance and therefore has never been reported as a cause of allergic contact dermatitis. Of course, an absence of clinical evidence means little if there has not been, over a period of years, a fair degree of exposure to tin.  Although data on exposure was not available to the report author, the complete absence of occupational skin disease in industries producing/using tin and the total absence of any consumer problems seems to be significant when one bears in mind that diagnostic patch testing began in the late 19th century and became common practice more than 50 years ago (Lachapelle, 2006).  In this context, the positive patch test results with tin II chloride should be mentioned.  Both the clinical significance of this data and its relevance to metallic tin is unknown.  However, since there are no sequelae to the work, and it seems quite probable that skin irritation played a significant part in the skin responses seen, then it is judged the data does not generate any material concern regarding skin sensitisation and metallic tin.

Lastly, does the other immunotoxicology data give any cause for concern regarding potential skin sensitisation hazard?  It is concluded that it does not.  Indeed, it could be argued that it positively suggests metallic tin is unlikely to be a skin sensitiser.  Plasma cells are not responsible for skin sensitisation (see the recent mechanistic review by Rustemeyer et al., 2006).  To add strength to this, it is considered important to note that the classic human metal skin sensitisers, nickel and chromium, both fail to produce responses of the kind generated by tin in certain rat strains.  If classic and common human allergens do not produce this response, it argues most powerfully that it is not an indication that metallic tin is a potential skin sensitiser.