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EC number: 227-813-5
CAS number: 5989-27-5
Table 1: Skin irritation data for individual
Mean (24 hours + 48 hours + 72 hours)
* marked desquamation from skin surface
after 7 days
** desquamation from skin surface after 7
In a primary dermal irritation study
performed according to OECD Guideline 404 and in compliance with GLP,
three albino rabbits were dermally exposed to 0.5 mL of undiluted
d-limonene, under a semi-occlusive patch for 4 hours to intact skin of
the flank. Animals were then observed for 7 days. Irritation was scored
by the method of Draize at 1, 24, 48 and 72 hours and 7 days after
Mean individual scores at 24, 48 and 72 h
after exposure for the 3 animals were 2, 2, 2 for erythema score and
1.33, 2, 1.33 for oedema score. Signs of erythema and oedema, as well as
desquamation from skin surface, were observed in all animals on Day 7.
In this study, d-limonene was found to be skin irritant when applied
topically to the rabbit.
Therefore, d-limonene is classified as ‘R38
Irritating to skin’ according to Directive 67/548/EEC but it is not
classified according to CLP Regulation (EC) No 1272/2008.
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