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EC number: 227-813-5 | CAS number: 5989-27-5
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- D-Limonene is one of the main constituents of dipentene multiconstituent (REACTION MASS OF BETA-PHELLANDRENE AND D-LIMONENE AND L-LIMONENE). Therefore, data on dipentene multiconstituent can be used for extrapolation to d-limonene. See read-across justification document in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 209 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 41 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 1 062 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The calculated respiration rates and inhibition percentages of dipentene are presented in Table II and the results of the graphical probit analysis are shown in Figure 2
- Results with reference substance (positive control):
- The EC50 of the reference compound was 7 mg/L which is within the prescribed range of 5 to 30 mg/L (Table I and Figure 1)
- Validity criteria fulfilled:
- yes
- Remarks:
- The control respiration rates were within 15% of each other; the EC50 of the reference compound was 7 mg/L
- Conclusions:
- The EC50 of dipentene multi-constituent for activated sludge after 3 hours contact time is 209 mg/L. The EC10, EC20 and EC80 are 18, 41 and 1062 mg/L, respectively
- Executive summary:
In a 3-hour acute toxicity study, activated sludge were exposed to dipentene multi-constituent at nominal concentrations of 0 (control), 8.45, 16.9, 33.8, 67.6, 135.2, 270.4, 540.8 mg/L under static conditions in accordance with Guidline OECD 209.
The EC50 and EC10 values based on respiration rate inhibition were 209 and 18 mg/L, respectively.
Therefore, dipentene multi-constituent is classified as not harmful to microorganisms and this study satisfies with the guidelines requirements for activated sludge toxicity study.
Reference
Table 6.1.7/1: Respiration rates of the activated sludge, inhibition percentages, and pH at various concentrations of 3,5-dichlorophenol after 3 hours contact time.
Concentration mg/L |
Activity mg O2/g.min |
Inhibition% |
pH start test |
pH end test |
Temperature end test (°C) |
Control |
0.282 |
-- |
6.8 |
7.0 |
20.6 |
Control |
0.253 |
-- |
6.8 |
7.0 |
20.3 |
Control* |
0.276 |
-- |
6.8 |
7.1 |
20.9 |
Control* |
0.259 |
-- |
6.8 |
7.1 |
21.1 |
1.5* |
0.235 |
12 |
6.8 |
6.9 |
21.0 |
3.0 |
0.165 |
38 |
6.9 |
7.2 |
20.4 |
5.9 |
0.135 |
49 |
6.9 |
7.2 |
20.7 |
11.8 |
0.106 |
60 |
6.9 |
7.2 |
20.4 |
23.6 |
0.053 |
80 |
6.9 |
7.2 |
20.8 |
47.2 |
0.035 |
87 |
6.9 |
7.1 |
7.1 |
* = Inhibition percentages at concentrations indicated by the asterisk are calculated with the controls indicated by the asterisk
Table 6.1.7/2: Respiration rates of the activated sludge, inhibition percentages and pH at various concentrations of dipentene after 3 hours contact time
Concentration mg/L |
Activity mg O2/g.min |
Inhibition% |
pH end test |
Temperature end test (°C) |
Control |
0.296 |
-- |
7.1 |
20.5 |
Control |
0.290 |
-- |
7.2 |
20.5 |
Control* |
0.313 |
-- |
7.2 |
20.8 |
Control* |
0.290 |
-- |
7.2 |
21.0 |
8.45* |
0.284 |
6 |
7.2 |
21.0 |
16.9* |
0.307 |
-2 |
7.1 |
20.9 |
33.8 |
0.238 |
19 |
7.2 |
20.4 |
67.6 |
0.174 |
41 |
7.3 |
20.3 |
135.2 |
0.133 |
54 |
7.4 |
20.3 |
270.4 |
0.122 |
58 |
7.4 |
20.5 |
540.8 |
0.133 |
54 |
7.4 |
20.5 |
* = Inhibition percentages at concentrations indicated by the asterisk are calculated with the controls indicated by the asterisk.
Description of key information
Data available on a structurally related substance, dipentene multiconstituent, show an EC50 of 209 mg/L and an EC10 of 18 mg/L
for toxicity to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 209 mg/L
- EC10 or NOEC for microorganisms:
- 18 mg/L
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