4,4'-methylenedianiline

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

validity criteria and details on results were not available, the study was performed according to OECD Guidelines and was generated by the Japanese Competent Authorities.

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Deviations:

not specified

Principles of method if other than guideline:

A bioconcentration test (May 1981) carried out on Carp (Cyprinus carpio)

GLP compliance:

not specified

Radiolabelling:

no

Details on sampling:

Sampling intervals / frequency for test organisms: every 2 weeks
Sampling intervals / frequency for test medium samples: twice a week

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Number of organisms per vessel: 15-20 fishes
Source: Sugishima fish farm (Kumamoto 866, Japan)
Length at study initiation: about 10 cm
Age at study initiation: no data
Weight at study initiation: about 30g
Feed during test: yes
Food type: pelleted feed for carp

Test type:

flow-through

Hardness:

no data

Test temperature:

25+/-2°C

pH:

7

Dissolved oxygen:

6-8 mg/l

Nominal and measured concentrations:

0.2ppm and 0.02ppm

Lipid content:

4.5 %

Remarks on result:

other: average

Conc. / dose:

200 µg/L

Type:

BCF

Value:

> 3 - < 14 dimensionless

Conc. / dose:

20 µg/L

Type:

BCF

Value:

ca. 3.1 - < 15 dimensionless

Details on results:

Test concentration: 0.2ppm
BCF: (3.0)-14

Test concentration: 0.02ppm
BCF: <3.1 - (15)

BCF results of <=15 indicate low potential for bioaccumulation. Using the Bcfwin (v2.7) model (based upon a study prepared for US EPA (Meylan et al., 1997)) with a measured log Kow value of 1.55, one obtains a predicted BCF value of 3.1 for 4,4'-MDA. This predicted BCF value is consistent with the experimental values obtained in the study.

Description of key information

 BCF values ranging from <3.1 to 15 were found for Cyprinus carpio after 42 days exposure. The test was performed according to OECD 305C.

Key value for chemical safety assessment

BCF (aquatic species):

15 dimensionless

Additional information

In an experimental study according to OECD 305 the bioaccumulative potential of the test istem was assessed in Cyprinus carpio. The test species was exposed to 200 µg/L and 20 µg/L over 42 d. No potential for bioaccumulation was observed with BCF values of 3-14 (200µg/L) and 3-15 (20 µg/L).