α-chlorotoluene

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP status of the study unknown. Study well documented and based on generally accepted scientific principles. This study should therefore be considered as a Klimisch 2.e study.

Data source

Reference

Reference Type:

publication

Title:

Sensory irritation caused by various industrial airborne chemicals

Author:

De Ceaurriz J.C., Micillino J.C., Bonnet P. and Guenier J.P.

Year:

1981

Bibliographic source:

Toxicology letters, 9, 137-143.

Materials and methods

Principles of method if other than guideline:

The authors the sensory iiration potential of industrial airborne chemicals on mice by assessing by analysing their respiratory rates with a plethysmograph.

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

respiratory irritation

Test material

Test material information

Constituent 1

Reference substance name:

α-chlorotoluene

EC Number:

202-853-6

EC Name:

α-chlorotoluene

Cas Number:

100-44-7

Molecular formula:

C7H7Cl

IUPAC Name:

(chloromethyl)benzene

Details on test material:

- Name of test material (as cited in study report): benzylchloride
- Physical state: liquid
- Analytical purity: high purity
No further inofrmation

Test animals

Species:

mouse

Strain:

Swiss

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Vehicle:

unchanged (no vehicle)

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

For about 5 minutes

Frequency of treatment:

one

Post exposure period:

No data

No. of animals per sex per dose:

Six male mice per concentration and four different concentrations

Results and discussion

Details on results:

The sensory irritation response measured was the average maximum percentage decrease in respiratory rate for six mice simultaneously exposed to vapor of the test chemical. The concentration that caused a 50 % decrease in the respiratory rate from baseline (RD 50) was calculated using least-squares regression analysis and gave a RD50 for benzyl chloride of 17 ppm.

Applicant's summary and conclusion

Conclusions:

In the test conditions, the authors tested the sensory irritation potential of benzyl chloride by following the decrease in the respiratory rate of mice exposed during five minutes (RD50) to vapours of the substance. They estimated a RD50 around 17 ppm for benzyl chloride.
However, at this level of information, no classification of specific target organ toxicity (here respiratory tract) could be done since the exposure period is only five minutes.

Executive summary:

The authors tested the sensory irritation potential of benzyl chloride (CAS n° 100 -44 -7) by following the decrease in the respiratory rate of six Swiss-OF1 mice (weighing 23 -27 g) exposed during five minutes (RD50) to vapours of the test substance. Respiratory frequency was monitored by a plethysmograph.

The concentration that caused a 50 % decrease in the respiratory rate from baseline was calculated using least-squares regression analysis and gave a RD50 for benzyl chloride of 17 ppm. This RD50 reflects the sensory irritation potential according to the authors.

No clear conclusion of the authors was drawn on the real sensory irritation potential of benzyl chloride. However, this value may help for a weight of evidence for a classification as a specific target organ toxicant (here respiratory tract) following single exposure category 3.

This study is well described and is based on recognized scientific principles. Therefore, this study should be considered as reliable with restrictions and acceptable for assessment.