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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
specific investigations: other studies
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP status of the study unknown. Study well documented and based on generally accepted scientific principles. This study should therefore be considered as a Klimisch 2.e study.

Data source

Reference
Reference Type:
publication
Title:
Sensory irritation caused by various industrial airborne chemicals
Author:
De Ceaurriz J.C., Micillino J.C., Bonnet P. and Guenier J.P.
Year:
1981
Bibliographic source:
Toxicology letters, 9, 137-143.

Materials and methods

Principles of method if other than guideline:
The authors the sensory iiration potential of industrial airborne chemicals on mice by assessing by analysing their respiratory rates with a plethysmograph.
GLP compliance:
not specified
Type of method:
in vivo
Endpoint addressed:
respiratory irritation

Test material

Constituent 1
Chemical structure
Reference substance name:
α-chlorotoluene
EC Number:
202-853-6
EC Name:
α-chlorotoluene
Cas Number:
100-44-7
Molecular formula:
C7H7Cl
IUPAC Name:
(chloromethyl)benzene
Details on test material:
- Name of test material (as cited in study report): benzylchloride
- Physical state: liquid
- Analytical purity: high purity
No further inofrmation

Test animals

Species:
mouse
Strain:
Swiss
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Vehicle:
unchanged (no vehicle)
Analytical verification of doses or concentrations:
yes
Duration of treatment / exposure:
For about 5 minutes
Frequency of treatment:
one
Post exposure period:
No data
No. of animals per sex per dose:
Six male mice per concentration and four different concentrations

Results and discussion

Details on results:
The sensory irritation response measured was the average maximum percentage decrease in respiratory rate for six mice simultaneously exposed to vapor of the test chemical. The concentration that caused a 50 % decrease in the respiratory rate from baseline (RD 50) was calculated using least-squares regression analysis and gave a RD50 for benzyl chloride of 17 ppm.

Applicant's summary and conclusion

Conclusions:
In the test conditions, the authors tested the sensory irritation potential of benzyl chloride by following the decrease in the respiratory rate of mice exposed during five minutes (RD50) to vapours of the substance. They estimated a RD50 around 17 ppm for benzyl chloride.
However, at this level of information, no classification of specific target organ toxicity (here respiratory tract) could be done since the exposure period is only five minutes.
Executive summary:

The authors tested the sensory irritation potential of benzyl chloride (CAS n° 100 -44 -7) by following the decrease in the respiratory rate of six Swiss-OF1 mice (weighing 23 -27 g) exposed during five minutes (RD50) to vapours of the test substance. Respiratory frequency was monitored by a plethysmograph.

The concentration that caused a 50 % decrease in the respiratory rate from baseline was calculated using least-squares regression analysis and gave a RD50 for benzyl chloride of 17 ppm. This RD50 reflects the sensory irritation potential according to the authors.

No clear conclusion of the authors was drawn on the real sensory irritation potential of benzyl chloride. However, this value may help for a weight of evidence for a classification as a specific target organ toxicant (here respiratory tract) following single exposure category 3.

This study is well described and is based on recognized scientific principles. Therefore, this study should be considered as reliable with restrictions and acceptable for assessment.