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EC number: 203-710-0 | CAS number: 109-83-1
- Life Cycle description
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- Density
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Additional ecotoxological information
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Test period: 05 Oct 1993 to 08 Oct 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Monomethylethanolamine
- Physical state: fluid
- Stability under test conditions: stable, not stable against acid solutions - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 12,5 and 0.78 mg/L, untreated control and stock solution (200 mg/L) after 0 h (unin) and 72 h
- Sampling method: initial concentration (0 h, uninoculated replicate); final concentration (72 h, uninoculated and inoculated sample after removal of algal cells through centrifugation) - Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Scenedesmus subspicatus CHODAT SAG 86.81
- Source (laboratory, culture collection): The used test strain of is purchased on regular times from the SAG (Sammlung Algenkulturen in Göttingen (Collection of algal culture in Göttingen, Germany) and kept in a liquid culture at the Laboratory of Ecology from BASF AG in Ludwigshafen, Germany.
- Age of inoculum (at test initiation): 72 h
- Method of cultivation: Stock cultures and precultures were kept in 250 mL erlenmeyers with 100 mL medium with an inoculation density of 10000 cells/mL. Stock cultures are weekly transferred, pre-cultures are incubated for 72h before use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 2° C
- pH:
- uninoculated samples, 0 h: 7.96 - 9.89
uninoculated samples, 72 h: 8.20 - 8.62
inoculated samples, 72 h: 8.55 - 9.65 - Nominal and measured concentrations:
- Nominal: 100, 50, 25, 12.5, 6.25, 3.13, 1.25, 0.78, 0.39 mg/L
Analytical recovery rates after 72 h: 88.8 - 103.0% in uninoculated samples, 88.8 - 108.0% in inoculated samples - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml Erlenmeyer, 100 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: uninoculated 0, 72 h; inoculated 72 h
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination, universal white light, L 25 (Osram)
- Light intensity and quality: 120 µE/(m²s)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber (Neubauer improved) at the timepoint, t=72h value.0 h-value is obtained from the inoculum
- Determination of fluorescence: At timepoints t=0, 24, 48, 72h, fluorescence was measured in a EOS filter fluorometer FL2. The in vivo chlorophyl-a fluorescence at 685 nm was determined as measure for biomass (pulsed excitation with light flashes having wavelengths of 435 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: no data - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 28.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 38.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 39.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- other: fluorescence
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (analytically verified)
- Basis for effect:
- other: fluorescence
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- - The EC values are calculated from the concentration-response relationship.
- The NOEC is determined by comparing the means of the fluorescence measurement of the various concentration levels with the control. The Duncan multiple range test is carried out at a 95% significance level . - Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 = 18.4 mg/L (effect: biomass)
- Executive summary:
In a GLP, 72-hour static test with the green alga Scenedesmus subspicatus according to EU method C.3, the algae toxicity of 2-methylaminoethanol was studied. Statistical evaluation of the endpoint growth rate revealed an EC50 (72h) of 28.1 mg/L, the NOEC was found to be 3.13 mg/L and the LOEC was found to be 6.25 mg/L, related to the analytically verified concentrations (BASF 1993). Furthermore, an EC10 value (72 h; growth inhibition) of 10.1 mg/L is reported.
Reference
Cell density (parameter fluorescence), mean of fluorometer units
Test item concentration (nominal; mg/L) |
Exposure duration (h) |
% of control (72 h) |
|||
0 |
24 |
48 |
72 |
||
Control |
71 |
231 |
1132 |
3601 |
100.0 |
100 |
64 |
22 |
3 |
6 |
0.2 |
50 |
63 |
59 |
38 |
26 |
0.7 |
25 |
63 |
176 |
697 |
800 |
22.2 |
12.5 |
64 |
202 |
1060 |
1862 |
51.7 |
6.25 |
64 |
211 |
1056 |
3141 |
87.2 |
3.13 |
65 |
204 |
1070 |
3402 |
94.5 |
1.56 |
69 |
209 |
1094 |
3630 |
100.8 |
0.78 |
63 |
207 |
1063 |
3387 |
94.1 |
0.39 |
67 |
204 |
1075 |
3610 |
100.2 |
Inhibition of cell growth and growth rate in comparison to the control after 72 hours
Test item concentration (nominal; mg/L) |
Biomass |
Growth rate |
||
Absolute |
Inhibition (%) |
Absolute |
Inhibition (%) |
|
control |
2915 |
----- |
0.055 |
----- |
100 |
-196 |
106.7 |
-0.033 |
160.3 |
50 |
-111 |
103.8 |
-0.012 |
122.5 |
25 |
1053 |
63.9 |
0.035 |
35.3 |
12.5 |
1969 |
32.5 |
0.047 |
14.2 |
6.25 |
2614 |
10.3 |
0.054 |
0.8 |
3.13 |
2748 |
5.7 |
0.055 |
-0.8 |
1.56 |
2877 |
1.3 |
0.055 |
-0.9 |
0.78 |
2743 |
5.9 |
0.055 |
-1.5 |
0.39 |
2850 |
2.2 |
0.055 |
-1.5 |
pH values at times t = 0 h and t= 72 h
Test item concentration (nominal; mg/L) |
pH values |
||
0 h |
72 h |
||
Uninoculated |
Uninoculated |
Inoculated |
|
control |
7.96 |
8.21 |
9.49 |
100 |
9.89 |
8.62 |
8.55 |
50 |
9.60 |
8.40 |
8.57 |
25 |
9.15 |
8.34 |
8.78 |
12.5 |
8.86 |
8.33 |
9.28 |
6.25 |
8.55 |
8.31 |
9.59 |
3.13 |
8.25 |
8.30 |
9.65 |
1.56 |
8.10 |
8.29 |
9.51 |
0.78 |
8.02 |
8.26 |
9.46 |
0.39 |
7.98 |
8.20 |
9.47 |
After neutralisation no appreciable reduction on harmful effects can be observed.
Summary of the concentration control analysis (detection limit = 1 mg/L)
Time |
Control |
Recovery (%) |
|
Test item solutions |
Stock solution |
||
0 h, uninoculated |
< 1 mg/L |
93.6 – 103.0 |
103.5 |
72 h, uninoculated |
< 1 mg/L |
88.8 – 103.0 |
105.5 |
72 h, inoculated |
< 1mg/L |
88.8 – 108.0 |
- |
Description of key information
N-Methylaminoethanol is regarded to be acutely harmful to algae (BASF AG, 1994).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 18.4 mg/L
- EC50 for marine water algae:
- 70 mg/L
- EC10 or NOEC for freshwater algae:
- 3.13 mg/L
Additional information
Scenedesmus subspicatus was used as test organism in a static freshwater toxicity test (BASF AG, 1994). The test was performed according to EU Method C.3 with a time duration of 72 hours. After this exposure time an EC50 of 28.1 mg/L, and an EC10 of 10.1 mg/L based on growth rate as well as an EC50 value of 18.4 mg/L, based on biomass was found. Regarding biomass, a NOEC of 3.13 mg/L, a 72 h (ErC10 = 5.97 mg/L, and a LOEC of 6.25 mg/L were reported (biomass; measured; pH 7.98 - 9.65). Concerning these results, N-Methylethanolamine can be regarded to be acutely harmful to algae.
In a screening study with marine phytoplankton species Skeletonema costatum according to ISO guideline 10253 (1999) an EC50 (72 h) value of 70 mg/L (growth rate) was calculated for MMEA.
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