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EC number: 203-710-0
CAS number: 109-83-1
Total number of mobile Daphnids after exposure (48 h) to various test
Test item conc.
Neutralised test samples showed no decrease
in toxic effects.
pH and oxygen values (measured in
Test item concentration
Dissolved oxygen after
Supporting analytical chemistry
Measured concentration (mg/L)
Stocked with daphnids
1 (1.3, 1.3)*
1 (1.3, 1.4)*
*: The concentrations found for these
samples are very close to the limit of determination. Therefore, the
accuracy of these data is less than the accuracy of the higher
As key study concerning acute toxicity
to freshwater invertebrates a GLP-test performed by BASF (1993)
according to EU methoc C.2 was identified, using Daphnia magna as test
species. In the static test an EC50 of 33 mg/L after 48 hours of
exposure was calculated related to the analytically measured
The key value for chemical safety assessment
is taken from an experimental result by BASF AG (1993).
Concerning acute toxicity to freshwater
invertebrates a GLP-test according to EU method C.2 is available (BASF
AG, 1993). Using Daphnia magna as test species in this static
test, an EC50 of 33 mg/L after 48 hours of exposure was determined. The
test concentrations (nominal and not ph adjusted: 0, 0.78, 1.56, 3.13,
6.25, 12.5, 25, 50 and 100 mg/L) were analytically verified. As the
recovery was between 90 % and 103 % the effect value was based on
48-hour EC50 was - as mentionned above - 33 mg/L (measured;
pH 8.0 – 9.9; 95% CI 30.0-36.2 mg/L). In this study, the pH values of up
to pH 9.9 were observed after 3 h, exceeding the tolerable pH-range of
pH 6.0 - pH 9.0. Therefore, the observed adverse effects may be partly
caused by a pH-shift to more alkyline pH-values at higher test substance
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