2-methylaminoethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: National procedure recommendation, no analytical monitoring

Qualifier:

according to guideline

Guideline:

other: National procedure recommendation F.1.1, Federal Environment Agency, 1982-06-01

Principles of method if other than guideline:

Acute toxicity test to fish

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Pre-Solution with of 5 g test substance and 2 g ethanol was dissolved with water for deriving stock solution.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): ethanol

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Length at study initiation (length definition, mean, range and SD): 30 ± 5 mm
- Feeding during test: no

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

ca. 15° dH

Test temperature:

20.4 - 20.8 °C

pH:

7.3 - 6.6

Dissolved oxygen:

7.0 - 9.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 8.4 L volume, 3.5 L fill volume
- Aeration: 3 bubles per minute
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: ca. 3 fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, filtered by activated carbon and ion exchange to remove Cu
- Ca/mg ratio: 4:1

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Test conditions

Exposure (h)	Temperature (°C)	Dissolved oxygen (mg/L)	pH
0	20.6	9.4	7.3
24	20.4	8.6	6.7
48	20.5	7.9	6.7
72	20.8	7.0	6.7
96	20.5	8.4	6.6

Validity criteria fulfilled:

yes

Conclusions:

LC50 > 100 mg/L

Executive summary:

An acute fish toxicity test was performed according to National procedure recommendation F.1.1, Federal Environment Agency, 1982-06-01. Danio rerio, previous name Brachydanio rerio was used as test species and the exposure time was 96 hours, giving a value LC50 > 100 mg/L.

Description of key information

The key value for chemical safety assessment is taken from two experimental results, which confirm: LC50 > 100 mg/L (Bayer AG, 1984) and LC50 = 100 mg/L (ICI Brixham Laboratory, 1986). 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

An acute fish toxicity test was performed according to the National procedure recommendation F.1.1., Federal Environmental Agency (Bayer AG, 1984). Brachydanio rerio was used as test species and the exposure time was 96 hours, giving a value LC50 > 100 mg/L. This result is confirmed by an experimental result performed by ICI Brixham Laboratory (1986) using Salmo gairdneri. In this case a LC50 (96h) of 100 mg/L is reported. Regarding these results, N-Methylethanolamine is not expected to be acutely harmful to fish.