Imidazolidine-2-thione

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for accessment.

Data source

Reference

Reference Type:

publication

Title:

Hepatic RNA synthesis in rats treated with ethylene thiourea

Author:

Austin GE, and Moyer GH

Year:

1979

Bibliographic source:

Res. Commun. Chem. Pathol. Pharmacol. 23: 639-642.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Animals (rats) received a single ip injection of ETU (2 doses) in DMSO.
One control group (DMSO).

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Imidazolidine-2-thione

EC Number:

202-506-9

EC Name:

Imidazolidine-2-thione

Cas Number:

96-45-7

Molecular formula:

C3H6N2S

IUPAC Name:

imidazolidine-2-thione

Details on test material:

ETU was a gift from Rohm and Haas, Co., Philadelphia, Pa.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Simonsen, Gilroy, Ca.
- Age at study initiation: no data
- Weight at study initiation: 130-200 g
- Fasting period before study: 16h
- Housing: no data
- Diet (e.g. ad libitum): of wayne meal
- Water (e.g. ad libitum): no data
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

DMSO

Details on exposure:

no

Doses:

2.5 and 250mg/kg

No. of animals per sex per dose:

4-7 animals / dose

Control animals:

yes

Details on study design:

[3H]orotic acid was given 60 min later ETU. Ninety minutes later this administration, the rats were decapited.
Then rates of hepatic RNA synthesis were determined.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD0

Effect level:

250 mg/kg bw

Remarks on result:

other: no toxicity observed

Mortality:

No data

Clinical signs:

No clinical signs of toxicity were seen in animals receiving a single injection of ETU (at low or high dosage).

Body weight:

No data

Gross pathology:

The livers of treated rats were grossly and histologically normal at time of sacrifice.

Other findings:

An injection of a low or high dose of ETU produces no significant alteration relative to controls in the pulse labeling of nuclear or cytoplasmic RNA.

Applicant's summary and conclusion

Conclusions:

In the present case, the lack of inhibition of RNA synthesis by ETU suggests that ETU and its metabolites may not alter cellular metabolism.

Executive summary:

Animals (rats) received a single ip injection of ETU (2 doses) in DMSO. DL0 was 250 mg/kg. In the present case, the lack of inhibition of RNA synthesis by ETU suggests that ETU and its metabolites may not alter cellular metabolism.