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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid
EC Number:
444-040-0
EC Name:
(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid
Cas Number:
52950-18-2
Molecular formula:
Hill formula: C8H7ClO3 CAS formula: C8H7CLO3
IUPAC Name:
(2R)-2-(2-chlorophenyl)-2-hydroxyacetic acid
Details on test material:
- Test substance: (R)-2-Chloromandelic acid
- purity: 99,7 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance was dissolved directly in test water

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: CIT, animals were cultured from eggs obtained from Viviers de France, Piscicultures des Fontaines, 64490 Sarrance, France.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
13-17 °C
pH:
7.37-7.82
Details on test conditions:
TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1g fish per L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered and dechlorinated potable water which is then softened by a Permo® water softening system.Half of the water passes directly into a holding tank attached to the flow-through system while the remaining half passes through a dejonizer producing ultra-pure water which is then fed into the holding tank. This results in a 1:1 dilution of potable dechlorinated:deiofliZed water giving a supply of die desirable hardness. The pH of die solution is between 6.0 and 8.5 and die total hardness is 150 ± 20 mgIL as CaCO3. The typical quality characteristics of this water are presented in appendix 2.

OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Other biological observations: One fish died while the range finding test in the concentration 10 mg/L. Due tto the fact, that in the limit test afterwards, no deaths occured in the highest concentration of 100 mg/L it is assumed to be an accidental death (i.e. not due to the test item)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes