Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid
EC Number:
444-040-0
EC Name:
(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid
Cas Number:
52950-18-2
Molecular formula:
Hill formula: C8H7ClO3 CAS formula: C8H7CLO3
IUPAC Name:
(2R)-2-(2-chlorophenyl)-2-hydroxyacetic acid
Details on test material:
- Test substance: (R)-2-Chlormandelsäure
- purity: 99.7 %
- aggregate state: solid/crystalline
- colour: white up to slightly yellowish

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from laboratory scale waste water plants fed with municipal sewage
- Pretreatment: none
- Concentration of sludge: 30 mg/L dry substance
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
39.5 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 +/- 2°C
- pH: 7.4-7.7
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: glass bootles
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: every 2 - 4 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes



STATISTICAL METHODS:
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
0 - 10
Sampling time:
28 d
Details on results:
Kinetic of control substance (in %):
= 10 after 3 day(s)
= 89 after 5 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed