(4-chlorophenyl)hydrazine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Only short abstract available.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Liver cell-mediated mutagenesis of V79 cells by hydrazine and related compounds.

Author:

Kuszynski C,

Year:

1981

Bibliographic source:

Environmental mutagenesis, 3:323–324; cited in WHO 2000, Phenylhydrazine. CICAD 19, Wissenschaftliche Verlagsgesellschaft mbH

Reference 2

Reference Type:

secondary source

Title:

Concise International Assessment Document 19: Phenylhydrazine

Author:

WHO

Year:

2000

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, ISBN 92 4 153019 7; ISSN 1020-6167

Reference 3

Reference Type:

secondary source

Title:

Unnamed

Year:

1994

Materials and methods

Principles of method if other than guideline:

The liver cell-mediated-V79 mutagenesis system (Langenbach et al. PNAS 75:2864-2867, 1978) with ouabain resistance as a marker was used. Cocultivation of the liver cells and V79 cells in the presence of carcinogens was shown to induce ouabain-resistant mutants of V79 cells. In the absence of liver cells, the carcinogens did not induce ouabain resistance.

GLP compliance:

no

Type of assay:

mammalian cell gene mutation assay

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): phenylhydrazine (PH)

Method

Target gene:

Na+K+-ATPase

Species / strain

Species / strain / cell type:

Chinese hamster lung fibroblasts (V79)

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

primar cultures of rat liver cells

Test concentrations with justification for top dose:

no data

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

N-dimethylnitrosamine

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium;

DURATION
- Exposure duration: 2 days
- Expression time (cells in growth medium): 2 days
- Selection time (if incubation with a selection agent): 12 - 14 days
- Fixation time (start of exposure up to fixation or harvest of cells): 16 - 18 days

SELECTION AGENT (mutation assays): Ouabain

Results and discussion

Test results

Test results 1

Species / strain:

Chinese hamster lung fibroblasts (V79)

Metabolic activation:

with

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

Chinese hamster lung fibroblasts (V79)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Phenylhydrazine was mutagenic only with primary liver cell activation.