2,2'-isopropylidenebis(p-phenyleneoxy)diethanol

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from December 12, 2001 to Febrary 28, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: In the study report there are no relevant deviation from the guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-isopropylidenebis(p-phenyleneoxy)diethanol

EC Number:

212-985-6

EC Name:

2,2'-isopropylidenebis(p-phenyleneoxy)diethanol

Cas Number:

901-44-0

Molecular formula:

C19H24O4

IUPAC Name:

2,2'-isopropylidenebis(p-phenyleneoxy)diethanol

Constituent 2

Reference substance name:

2-[4-[2-[4-(2-hydroxyethoxy)phenyl]propan-2-yl]phenoxy]ethanol

IUPAC Name:

2-[4-[2-[4-(2-hydroxyethoxy)phenyl]propan-2-yl]phenoxy]ethanol

Constituent 3

Reference substance name:

O(CCO)c1ccc(cc1)C(c1ccc(OCCO)cc1)(C)C

IUPAC Name:

O(CCO)c1ccc(cc1)C(c1ccc(OCCO)cc1)(C)C

Details on test material:

Lot.No.07704, purity: 80%

Radiolabelling:

no

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

The test sample (purity 80%, 500mg) was soluble in acetonitril with HCO-40 (10g). After removal of acetonitril by evaporation, it was added to purified water and adjusted to 2000mL. The solution was diluted with purified water for each test solution.

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

0 d

Test conditions

Test temperature:

24.1-25.0

pH:

7.3-7.7

Dissolved oxygen:

7.0-8.2mg/L

Nominal and measured concentrations:

Nominal: 4 micro gram/L measured (mean): 3.84 micro gram/L
Nominal: 40 micro gram/L measured (mean): 38.5 micro gram/L

Reference substance (positive control):

no

Details on estimation of bioconcentration:

BCFss = (Cf-CfB) / Cw
BCFss: bioconcentration factor (steady-state)
Cf: concentration of the test substance in/on the fish
CfB: concentration of the test substance in/on the fish exposured negative control (fresh water)
Cw: concentration of the chemical in the surrounding medium

Results and discussion

Lipid content

Lipid content 1

Lipid content:

2.79 %

Time point:

start of exposure

Lipid content 2

Lipid content:

2.61 %

Time point:

end of exposure

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

< 41.7

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:4 micro gram/L

Bioaccumulation factor 2

Type:

BCF

Value:

< 4.6

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:40 micro gram/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Low bioconcentration

Executive summary:

The test fishes did not show any abnormal activity and form.

The mean concentrations of test solutions were 3.84 and 38.5 micro gram per liter, they were stable and almost according to nominal concentrations.

The test substance concentrations in test fishes were measured on day 14, 18, 21, 25 and 28, these values were stable. The BCFss were estimated < 41.7 and < 4.6 at low and high exposure levels respectively.

The BCFss values were lower than the values of high bioaccumulation substances, which were polychlorobiphenyl, hexachlorobenzene and polychloronaphthalene. Therefore the bioaccumulation of test substance was estimated to be low.