2,2'-bipyridyl

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

One drop of saline containing the test item was placed in the conjunctival sac of the right eye of three albino rabbits, the left eye serving as control. Ocular lesions were rated according to the Draize scoring system.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-bipyridyl

EC Number:

206-674-4

EC Name:

2,2'-bipyridyl

Cas Number:

366-18-7

Molecular formula:

C10H8N2

IUPAC Name:

2,2'-bipyridine

Details on test material:

- Name of test material (as cited in study report): 2,2'-bipyridyl
- Substance type: yellowish-fawn crystalline solid
- Physical state: solid

Test animals / tissue source

Species:

rabbit

Strain:

other: albino

Test system

Vehicle:

physiological saline

Controls:

not required

Duration of treatment / exposure:

Single exposure.

Observation period (in vivo):

The observation period was 7 days

Number of animals or in vitro replicates:

Three animals were used.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of three animals

Time point:

other: 1-2 h

Score:

8

Reversibility:

fully reversible within: 5 d

Results 2

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of three animals

Time point:

24 h

Score:

3

Reversibility:

fully reversible within: 5 d

Results 3

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of three animals

Time point:

48 h

Score:

1

Reversibility:

fully reversible within: 5 d

Results 4

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of three animals

Time point:

other: 5 d

Score:

0

Reversibility:

fully reversible within: 5 d

Results 5

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of three animals

Time point:

7 d

Score:

0

Reversibility:

fully reversible within: 5d

Results 6

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of three animals

Time point:

other: 1-2 hours

Score:

0

Reversibility:

fully reversible within: 5 d

Results 7

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of three animals

Time point:

24 h

Score:

0

Reversibility:

fully reversible within: 5 d

Results 8

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of three animals

Time point:

48 h

Score:

0

Reversibility:

other: no irritation

Results 9

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of three animals

Time point:

other: 5 d

Score:

0

Reversibility:

other: no irritation

Results 10

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of three animals

Time point:

7 d

Score:

0

Reversibility:

other: no irritation

Results 11

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of three animals

Time point:

other: 1-2 h

Score:

8

Reversibility:

fully reversible within: 5 d

Results 12

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of three animals

Time point:

24 h

Score:

3

Reversibility:

fully reversible within: 5 d

Results 13

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of three animals

Time point:

48 h

Score:

1

Reversibility:

fully reversible within: 5 d

Results 14

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of three animals

Time point:

other: 5 d

Score:

0

Results 15

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of three animals

Time point:

7 d

Score:

0

Results 16

Irritation parameter:

iris score

Basis:

mean

Remarks:

of three animals

Time point:

other: 1-2 h

Score:

0

Results 17

Irritation parameter:

iris score

Basis:

mean

Remarks:

of three animals

Time point:

24 h

Score:

0

Results 18

Irritation parameter:

iris score

Basis:

mean

Remarks:

of three animals

Time point:

48 h

Score:

0

Results 19

Irritation parameter:

iris score

Basis:

mean

Remarks:

of three animals

Time point:

other: 5 d

Score:

0

Results 20

Irritation parameter:

iris score

Basis:

mean

Remarks:

of three animals

Time point:

7 d

Score:

0

Irritant / corrosive response data:

Irritation response data after application of 2,2'-bipyridine are summarized in table 1.

Any other information on results incl. tables

Table 1: Eye irritation data after instillation of 2,2’-bipyridine into rabbit eyes

Parameter	1-2 h	d 1	d 2	d 5	d 7
Cornea	0	0	0	0	0
Iris	0	0	0	0	0
Conjunctivae	8	3	1	0	0
Mean total score	8	3	1	0	0

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

Instillation of 2,2'-bipyridine into rabbit eyes resulted in moderate initial pain followed by infection and chemosis of the conjunctivae. Effects were reversible within 5 days.

Executive summary:

Eye irritating properties of 2,2’-bipyridine were tested in an in vivo test according to the method of Draize (1959). Briefly, one drop of a solution of the test item in saline was applied into the conjunctival sac of the right eye of three albino rabbits, the left eye serving as untreated control. There was no information on GLP nor were closer information on the scoring system or the amount of test item applied into the eyes available.

Instillation of 2,2'-bipyridine into rabbit eyes resulted in moderate initial pain followed by some infection and chemosis of the conjunctivae which was reversible within 5 days.