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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972
Report date:
1972

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
One drop of saline containing the test item was placed in the conjunctival sac of the right eye of three albino rabbits, the left eye serving as control. Ocular lesions were rated according to the Draize scoring system.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-bipyridyl
EC Number:
206-674-4
EC Name:
2,2'-bipyridyl
Cas Number:
366-18-7
Molecular formula:
C10H8N2
IUPAC Name:
2,2'-bipyridyl
Details on test material:
- Name of test material (as cited in study report): 2,2'-bipyridyl
- Substance type: yellowish-fawn crystalline solid
- Physical state: solid

Test animals / tissue source

Species:
rabbit
Strain:
other: albino

Test system

Vehicle:
physiological saline
Controls:
not required
Duration of treatment / exposure:
Single exposure.
Observation period (in vivo):
The observation period was 7 days
Number of animals or in vitro replicates:
Three animals were used.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Remarks:
of three animals
Time point:
other: 1-2 h
Score:
8
Reversibility:
fully reversible within: 5 d
Irritation parameter:
overall irritation score
Basis:
mean
Remarks:
of three animals
Time point:
24 h
Score:
3
Reversibility:
fully reversible within: 5 d
Irritation parameter:
overall irritation score
Basis:
mean
Remarks:
of three animals
Time point:
48 h
Score:
1
Reversibility:
fully reversible within: 5 d
Irritation parameter:
overall irritation score
Basis:
mean
Remarks:
of three animals
Time point:
other: 5 d
Score:
0
Reversibility:
fully reversible within: 5 d
Irritation parameter:
overall irritation score
Basis:
mean
Remarks:
of three animals
Time point:
7 d
Score:
0
Reversibility:
fully reversible within: 5d
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
of three animals
Time point:
other: 1-2 hours
Score:
0
Reversibility:
fully reversible within: 5 d
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
of three animals
Time point:
24 h
Score:
0
Reversibility:
fully reversible within: 5 d
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
of three animals
Time point:
48 h
Score:
0
Reversibility:
other: no irritation
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
of three animals
Time point:
other: 5 d
Score:
0
Reversibility:
other: no irritation
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
of three animals
Time point:
7 d
Score:
0
Reversibility:
other: no irritation
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
of three animals
Time point:
other: 1-2 h
Score:
8
Reversibility:
fully reversible within: 5 d
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
of three animals
Time point:
24 h
Score:
3
Reversibility:
fully reversible within: 5 d
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
of three animals
Time point:
48 h
Score:
1
Reversibility:
fully reversible within: 5 d
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
of three animals
Time point:
other: 5 d
Score:
0
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
of three animals
Time point:
7 d
Score:
0
Irritation parameter:
iris score
Basis:
mean
Remarks:
of three animals
Time point:
other: 1-2 h
Score:
0
Irritation parameter:
iris score
Basis:
mean
Remarks:
of three animals
Time point:
24 h
Score:
0
Irritation parameter:
iris score
Basis:
mean
Remarks:
of three animals
Time point:
48 h
Score:
0
Irritation parameter:
iris score
Basis:
mean
Remarks:
of three animals
Time point:
other: 5 d
Score:
0
Irritation parameter:
iris score
Basis:
mean
Remarks:
of three animals
Time point:
7 d
Score:
0
Irritant / corrosive response data:
Irritation response data after application of 2,2'-bipyridine are summarized in table 1.

Any other information on results incl. tables

Table 1: Eye irritation data after instillation of 2,2’-bipyridine into rabbit eyes

Parameter

1-2 h

d 1

d 2

d 5

d 7

Cornea

0

0

0

0

0

Iris

0

0

0

0

0

Conjunctivae

8

3

1

0

0

Mean total score

8

3

1

0

0

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Conclusions:
Instillation of 2,2'-bipyridine into rabbit eyes resulted in moderate initial pain followed by infection and chemosis of the conjunctivae. Effects were reversible within 5 days.
Executive summary:

Eye irritating properties of 2,2’-bipyridine were tested in an in vivo test according to the method of Draize (1959). Briefly, one drop of a solution of the test item in saline was applied into the conjunctival sac of the right eye of three albino rabbits, the left eye serving as untreated control. There was no information on GLP nor were closer information on the scoring system or the amount of test item applied into the eyes available.

Instillation of 2,2'-bipyridine into rabbit eyes resulted in moderate initial pain followed by some infection and chemosis of the conjunctivae which was reversible within 5 days.