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EC number: 206-674-4
CAS number: 366-18-7
The test item 2,2'-bipyridine slightly increased rat liver CYP content
in the low-dose group (15.6 mg/kg bw) but decreased CYP content with
increasing dose levels. In the Western Blot analysis, 2,2'-bipyridine
showed no effect neither on CYP 1A1/2 nor on CYP 2B1/2 expression.
The activity of the drug-metabolizing enzymes aminopyrine
demethylase, aniline hydroxylase, and dimethylnitrosamine demethylase
was slightly but significantly increased at lower dose levels (15.6
-31.2 mg/kg bw) but declined at higher concentrations.
Heme oxygenase activity and GSH content of rat liver was significantly
increased in a dose-dependant manner after treatment with
2,2'-bipyridine. Experiments with cycloheximide (an inhibitor of protein
biosynthesis) revealed that increased enzyme activity was due to de novo
protein synthesis (data not shown).
Table 1: Dose response of 2,2’-bipyridine on drug-metabolizing
enzyme activities, heme oxygenase activity and GSH content in rat liver,
mean ± SD of 4-6 rats.
Aminopyrine demethylase [nmol/mg protein/min]
Aniline hydroxylase [nmol/mg protein/min]
Dimethylnitrosamine demethylase [nmol/mg protein/min]
Heme oxygenase [nmol/mg protein/h]
GSH content [µmol/g liver]
Male Wistar rats received a single i.p. injection of the test item
2,2'-bipyridine (15.6-125.0 mg/kg bw) dissolved in corn oil and were
decapitated 24 h later. Glutathione content was determined from liver
which was cut off before liver perfusion was performed. Cytochrome P450
content and activity of different enzymes were measured from liver cell
Administration of 2,2'-bipyridyl in corn oil to rats via i.p. injection
resulted in a concentration-dependant increase of hepatic heme oxygenase
activity and glutathione content. The content of cytochrome P450 and of
the drug-metabolizing enzymes aminopyrine demethylase, aniline
hydroxylase, and dimethylnitrosamine demethylase firstly increased but
then declined at higher concentrations.
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