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EC number: 214-275-1
CAS number: 1119-34-2
The cell viability was measured by determining the optical density (OD)
at a wavelength of 540 nm. An exposure time of 20 minutes was employed.
The mean viability of the cells exposed to the test item was 98.0% of
the mean negative control value. The OD540 values were well above the
cut-off percentage cell viability value that distinguishes irritant from
non-irritant test items of >50% for a 20-minute exposure.
The test item was considered to be non-cytotoxic and predicted to be not
irritant to skin.
The viability of cells treated with the positive reference item, 5% SDS,
was 6.9% of the negative controls and was below the cut-off value.
Hence, 5% SDS is predicted to cause pronounced skin irritation.
All quality criteria required were fulfilled.
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