Phosphoric acid, isononyl ester

Endpoint summary

Additional information

No reliable data are available for the aquatic toxicity of isononyl phosphate to invertebrates, fish, algae, or microorganisms. However, reliable short-term data for fish and invertebrates, and microorganisms and data for algae are available for the analogue, Phosphoric acid, 2-ethylhexyl ester (CAS 12645 -31 -7) and are included in the dossier.

One reliable key study, Goodband & Mullee (2011), with the analogue,Phosphoric acid, 2-ethylhexyl ester,

is submitted for the short-term toxicity to fish endpoint. The study was conducted using a standard approach for testing poorly soluble, multi-component substances, the preparation of a water accommodated fraction (WAF). As such, the effect values are presented as loading rates (e.g. LL₅₀or EL₅₀), and not measured aqueous concentrations. The 96hr LL50 = >100 mg/L.

No reliable data are available for the short-term aquatic toxicity of isononyl phosphate to aquatic invertebrates. However, reliable data are available for the analogue,Phosphoric acid, 2-ethylhexyl ester (CAS 12645 -31 -7) and are included in the dossier.

Three key studies are submitted for the short-term toxicity to aquatic invertebrates endpoint, one for a freshwater species and two for marine species. Each study was conducted using a standard approach for testing poorly soluble, multi-component substances, the preparation of a water accommodated fraction (WAF). As such, the effect values are presented as loading rates (e.g. LL₅₀or EL₅₀), and not measured aqueous concentrations. Goodband & Mullee (2011) provided a 48h EL50 = >100 mg/L for Daphnia magna. Furst (2002) provided a 48hr LL50 of 117.4 mg/L for the marine invertebrate, Acartia tonsa.

One reliable key study, Vryenhoef & Mullee (2011), is submitted for the toxicity to aquatic algae endpoint, one for a freshwater species. The study was conducted using a standard approach for testing poorly soluble, multi-component substances, the preparation of a water accommodated fraction (WAF). As such, the effect values are presented as loading rates (e.g. EL₅₀or NOELr), and not measured aqueous concentrations. The 72h EL50 (growth rate) = 49 mg/L, 72h NOELr (growth rate) = 25 mg/L for Pseudokirchnerella subcapitata.

A study Clarke (2011) was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test", Method C.11 of Commission Regulation (EC) No. 440/2008 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.6800. The 3-Hour EC₅₀was 420 mg/l (95% confidence limits 330 - 530). The No Observed Effect Concentration (NOEC) after 3 hours exposure was150mg/l.