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EC number: 931-338-5
CAS number: 90622-77-8
The growth rate over the two-day expression
period for cultures treated with DMSO was between 13 and 23 (3 h
treatment) and 37 and 38 (24 h treatment). Mutation frequencies in
cultures treated with positive control chemicals were increased by 8.2
and 16-fold for MMS in the absence of S9-mix, and by 10- and 13-fold for
CP in the presence of S9-mix, in the first and second experiment
respectively. It was therefore concluded that the test conditions, both
in the absence and presence of S9-mix, were appropriate for the
detection of a mutagenic response and that the metabolic activation
system (S9-mix) functioned properly. In addition the observed mutation
frequencies of the positive control substances were within the
acceptability criteria of this assay.
study was conducted to evaluate the in vitro genetic toxicity
of the test substance, C8-18 and C18-unsatd. MEA (>95% active) according
to OECD Guideline 476 and EU method B.17, in compliance with GLP. The
test was performed in two independent experiments with L5178Y mouse
lymphoma cells, in the absence and presence of S9-mix. In the first
experiment, the substance was tested up to concentrations of 60 and 200
µg/mL in the absence and presence of 8% (v/v) S9-mix. The incubation
time was 3 h. Test material was tested up to cytotoxic levels of 74 and
77% in the absence and presence of S9-mix, respectively. In the second
experiment, test material was tested up to concentrations of 45 and 225
µg/mL in the absence and presence of 12% (v/v) S9-mix with incubation
times of 24 and 3 h, respectively. The substance was tested up to the
cytotoxic level of 95% (absence of S9-mix) and up to 72% (presence of
S9-mix), but failed to induce a significant increase in the frequency of
mutations. The spontaneous mutation frequencies in the solvent-treated
control cultures were within historical control data range and therefore
within the acceptability criteria of the assay. Mutation frequencies in
positive control cultures were elevated 8.2- and 16-fold for MMS
(absence of S9-mix), and 10- and 13-fold for CP (presence of S9-mix).
Negative results were confirmed in an independent repeat experiment with
extended exposures. Under the study conditions, the test substance was
not mutagenic in the TK mutation test system both with and without
metabolic activation (Verspeek-Rip, 2009).
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