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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25.01 - 12.03.2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Vehicle:
no
Details on test solutions:
The solutions were prepared by diluting the stock solution (5000 mg/L) (volumetric pipette / volumetric flask for intermediate stock solutions of concentration 1000 and 100 mg/L). The solutions were prepared by measuring the required amount of stock solution with an automatic pipette and filling up in a measuring cylinder.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia magna STRAUS, line Berlin
- Age: 1 - 24 h

Maintenance
Daphnia magna is routinely maintained at LAUS GmbH and grown by parthenogenesis for toxicity tests.
- Breeding tubes: Beakers, nominal volume 2 L
- Medium: M4 medium after ELENDT
- Food: fish food Tetra Animin, finely ground, in M4 medium suspended and boiled up, unicellular green algae (Scenedesmus subspicatus)
- Transfer: twice / week
- Light: 16/8 h/d
- temperature: 20 +- 2°C
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 d
Test temperature:
20 +- 2°C
pH:
7.8 +- 2
Dissolved oxygen:
> 4 mg/L
Nominal and measured concentrations:
Nominal concentration [mg/L]: 5000, 2500, 1250, 625, 320, 160, 80, 40, 20, control
Details on test conditions:
The test beakers were left for 24 hours without feed addition and illumination. Thereafter, the count of immobile daphnids was done. The vessels were swiveled lightly. If the animals showed no motion or merely moved the feelers slightly, they were paid as immobilized.
The pH and oxygen concentration in the test vessels were measured at the beginning and end of the test.
- test duration: 24 h
- test temperatur: 20 +- 2°C
- test vessels: beaker with 500 mL nominal volume
- 4 test vessels / concentration a 20 mL with 5 daphnids each
- control: 4 test vessels a 20 mL with 5 daphnids each
- nine concentrations (5000, 2500, 1250, 625, 320, 160, 80, 40, 20 mg/L)
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
300 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 120 - 800 mg/l
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
2 500 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The 24h-EC50 of potassium dichromate towards daphnids was determined as 1.82 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
The 24h-EC50 of the test item was determined as 300 mg/L.
Executive summary:

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure according to OECD 202. The range-finding test showed a 24 EC50 value between 100 and 1000 mg/L.

In the final EC50 test Daphnia were therefore exposed for a maximum of 24 hours to nine different concentrations (5000, 2500, 1250, 625, 320, 160, 80, 40, 20 mg/L). The study included a blank-control. The test was performed in quadruplicate with 5 daphnia per vessel. The 24h EC50 was determined as 300 mg/L.

The 24 h EC50 of the reference substance was determined as 1.82 mg/L.

Description of key information

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure according to OECD 202. The range-finding test showed a 24 EC50 value between 100 and 1000 mg/L.

In the final EC50 test Daphnia were therefore exposed for a maximum of 24 hours to nine different concentrations (5000, 2500, 1250, 625, 320, 160, 80, 40, 20 mg/L). The study included a blank-control. The test was performed in quadruplicate with 5 daphnia per vessel. The 24h EC50 was determined as 300 mg/L.

The 24 h EC50 of the reference substance was determined as 1.82 mg/L.

Key value for chemical safety assessment

Additional information