Registration Dossier
Registration Dossier
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EC number: 423-460-8 | CAS number: 3508-98-3 SALICYNALVA
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
A study according to OECD 301D was performed to assess the ready biodegradability of Salicynalva. Salicynalva attained no biodegradation after 28 days and may not be termed as readily biodegradable. Salicynalva was not found to be inhibitory to activated sewage sludge bacteria under the conditions of this test.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
Additional information
A study was performed to assess the ready biodegradability of Salicynalva. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (0.1. No. L383A, 29.12.92) Part C, Method 4-E "Determination of ready biodegradability, Closed Bottle Test" and the OECD Guideline for Testing of Chemicals No. 301D "Ready Biodegradability: Closed Bottle Test". The test substance (purity > 95%) was dissolved in chloroform to give a stock solution of 560 mg/10 mL. Then, 10 μL aliquots of stock solution were placed on individual pieces of glass filter paper and the solvent allowed to evaporate to dryness. Sealed bottles containing the test substance (adsorbed onto glass filter paper) and inorganic nutrient medium were inoculated with activated sewage sludge bacteria (collected from a sewage treatment plant predominantly receiving domestic sewage) and incubated for up to 28 days at 20 ± 1°C. On Days 0, 4, 7, 11, 14, 18, 21, 25 and 28 duplicate bottles were taken and dissolved oxygen measurements were performed electrochemically. Percentage biodegradation values were determined by comparing the extent of oxygen depletion with the Theoretical Oxygen Demand (2.77 mg O2/mg). Additional bottles, containing both the test substance and a readily biodegradable standard substance were prepared in order to provide additional information on the inhibitory effect of the test substance. Salicynalva attained no biodegradation after 28 days and may not be termed as readily biodegradable. Salicynalva was not found to be inhibitory to activated sewage sludge bacteria under the conditions of this test.
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