Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 278-047-3
CAS number: 74993-03-6
Two acute oral toxicity studies were conducted on C4 Amin. The result of the studies were:- The rat oral LD50 is greater than 200 mg/kg and less than 2,000 mg/kg when tested according to OECD 423.- The rat oral LD50 is 540 mg/kg when tested according to a protocol developed by Litchfield and Wilcoxon.
The acute oral toxicity potential of the test article was evaluated in
male and female rats. The test was not intended to comply with GLP
guidelines. The test method was based on EEC Guidelines B.1 (1996) and
OECD 423 (1996). Male and female rats (Wistar, 3/sex/group) received 200
mg/kg test article suspended in bi-distilled water via single oral
gavage at a dose volume of 10 mL/kg. Three additional female rats
received 2,000 mg/kg test article in the same manner. Rats were observed
at 1, 2, 3 and 5 hours post-dose, and once daily for 14 days for
clinical signs of toxicity. Body weights were recorded on Day 1 (prior
to administration) and on Days 8 and 15. All animals were examined via
necropsy at termination. All animals (3 females) dosed at 2,000 mg/kg
were found dead 5 or 7 hours after treatment. Ruffled fur, hunched
posture, lateral recumbency, tremors and half closed lids were noted in
the female rats dosed at 2000 mg/kg prior to death. The animals dosed at
200 mg/kg survived through the end of the observation period and had no
abnormal clinical signs. Black-brown contents in the stomach, duodenum
and jejunum were noted in all females dosed with 2000 mg/kg test article
upon necropsy. All other animals were without macroscopic findings. The
body weight gain of the animals in the 200 mg/kg group was within the
range commonly recorded for this strain and age. Based on the results of
the test, the oral LD50 of the test article is > 200 mg/kg body weight
(LD0) and < 2,000 mg/kg body weight (LD100).
The acute oral toxicity potential of the test article (CASRN 74993-03-6,
Clear colorless liquid, Purity: >95%, Density: 1 g/mL, Internal sample
number A. Nr. 00267) was evaluated in male and female Wistar rats. The
study information presented below is based on a report with limited
data. The test was not conducted according to GLP guidelines. The test
method was based on an acute toxicity protocol developed by Litchfield
and Wilcoxon (1949). Male and female rats (5/sex/group) received 0.422,
0.51, 0.62, 0.75, or 0.91 mL/kg (422, 510, 622, 750, or 910 mg/kg) test
article at a dose volume of 10 mL/kg. Animals were observed for 14 days
post-dose for mortality and clinical signs. At 370 mg/kg one male (1/5)
died between Days 2 and 7. At 510 mg/kg, three males (3/5) died between
Days 2 and 7. At 622 mg/kg, three males (3/5) and two females (2/5) died
between Days 2 and 7. At 750 mg/kg, four males (4/5) and three females
(3/5) died during the 14 Day observation period. At 910 mg/kg, all males
(5/5) and four females (4/5) died during the 14 day observation period.
All other animals survived to the end of the observation period (Day
14). The study report provided limited details on clinical signs of
toxicity. The clinical signs were summarized and were not presented on a
group or animal basis. Based on the available information, observed
clinical signs included cowered position, piloerection, ptosis,
incrustation of the nose and eyes, apathy, and loss of weight. The
report indicates that clinical signs decreased after Study Day 4;
however, it did not indicate when or if clinical signs fully resolved
through Study Day 14. Based on the results of the study, the oral LD50
of the test article is 622 mg/kg body weight.
The test article meets the CLP classification criteria for Acute
Toxicity Category 4.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again