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EC number: 278-047-3
CAS number: 74993-03-6
Toxicity of C4-Amin to D. magna was assessed in a limit test conducted
according to OECD 202. The test substance was not fully soluble at the
limit of 100 mg/L, so a water soluble fraction (WSF) was made. Test
substance (100 mg/L) was sonicated in reconstituted test water for 15
minutes, stirred for 48 hours, filtered to 0.45 µm under reduced
pressure, and adjusted for pH. A 1:2 dilution series was tested of the
WSF stock. Test substance concentrations were not analytically
determined. Immobilization at 48 hours was 100% in the undiluted stock
and 25% in the 1:2 dilution. No other dilutions displayed any
While a toxic effect was observed in this study, it is not appropriate
to calculate effects concentrations using nominal concentrations from a
dilution series of a water soluble fraction when actual concentration of
the WSF is not known. Further, the test substance should have been fully
soluble at the concentration tested. This test is considered
unreliable. It is not suitable for Risk Assessment, Classification &
Labeling, and PBT Analysis.
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