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EC number: 229-851-8
CAS number: 6786-83-0
Table 1- The above table indicates,
eight reference substances were taken according to structural
similarity with the test chemical to prepare calibration graph.
Individual reference substances were allowed to pass through the
column on fixed operating conditions in order to obtain the values of tRand
SD. These values are then used to determine k' which is then
converted to log k' value (applying equation no. 1). This log k' was
then plotted on the calibration curve to determined log Koc value of
The adsorption coefficient Koc in soil
and in sewage sludge of test chemical was determined by the Reverse
Phase High Performance Liquid Chromatographic method according to OECD
Guideline No. 121 for testing of Chemicals. A test item solution was
prepared by accurately weighing 20 mg of test item and diluted with ACN
up to 10 ml. Thus, the test solution concentration was 2000 mg/l. The
sample was sonicated for 2 minutes and filtered through a 0.22 µm
syringe filter. The pH of the sample was found to be 7.9. Each of the
reference substance and test substance were analysed by HPLC at 210 nm.
For the HPLC method, HPLC model no. Agilent Technologies,1260 Infinity
II was used. Column used was Waters sperisorb, 4.6*250 mm, 5µ (CL-25).
Acetonitrile : water (55:45) was used as a mobile phase. During the
study, flow rate was 1.0ml/min. Study was performed at a temperature of
25°C. Injector volume was 10μl. Chem Station Open lab Control Panel was
used as a data Acquisition software and detector wavelength taken for
the study was 210 nm. After equilibration of the HPLC system, Urea was
injected first, the reference substances were injected in duplicate,
followed by the test chemical solution in duplicate. Reference
substances were injected again after test sample, no change in retention
time of reference substances was observed. Retention time tR were
measured, averaged and the decimal logarithms of the capacity factors k'
were calculated. The graph was plotted between log Koc versus log k'.
The linear regression parameter of the relationship log Koc vs log k'
were also calculated from the data obtained with calibration samples and
therewith, log Koc of the test substance was determined from its
measured capacity factor. The reference substances were chosen according
to estimated Koc range of the test substance and generalized calibration
graph was prepared. The reference substances Aniline, 4-Chloroaniline,
2,5-Dichloroaniline, Naphthalene, Biphenyl, Phenanthrene, Triphenylamine
and DDT were chosen having log Koc range from 1.8 to 5.4. The Log Koc
value of test chemical was determined to be 6.475±0.010 at 25°C.This log
Koc value indicates that the test chemical has a very strong sorption to
soil and sediment and therefore have negligible migration potential to
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