Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 482-140-6 | CAS number: 13641-96-8
Ecotoxicological information
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 Mar 2011 - 25 Mar 2011
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Because no significant phytotoxicity was showed in the range-finding test (p<0.05), no definitive testing was carried out.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.4200 (Seed Germination/Root Elongation Toxicity Test)
- Version / remarks:
- 1996
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 482-140-6
- EC Name:
- -
- Cas Number:
- 13641-96-8
- Molecular formula:
- Hill formula: C6 H7 N O3 CAS formula: C6 H7 N O3
- IUPAC Name:
- 2-isocyanatoethyl prop-2-enoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Fresh test solutions were added to glass bottles that have been filled with 100 g clean quartz sand.
- The test solution of AOI was prepared by directly adding appropriate amount of the test item into distilled water.
Test organisms
open allclose all
- Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Corn
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Daucus carota
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Carrot
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- other: Sesamum indicum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Sesame
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- other: Vigna radiate
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Mung bean
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Triticum aestivum
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Common wheat
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Brassica oleracea var. capitata
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cabbage
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Soybean
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Lettuce
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Cucumber
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
- Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Tomato
- Source of seed: Nanjing Research lnstitute of Vegetables and Flowers
- Prior seed treatment/sterilization: Only untreated seed (not treated with fungicides, repellents, etc.)
- Seed storage: coId storage (5 °C) in moisture-proof containers; seed moisture < 10%
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Test temperature:
- 24 - 25 °C
- pH:
- 7.4 - 7.8
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Glass bottles
- Amount of soil: 100 g quartz sand
- Method of seeding: The seed was positioned on the substrate allowing adequate room for anticipated growth.·The radicle end of the seed was aligned in the direction of this growth.
- No. of seeds per container: 15
- No. of replicates per treatment group: 1
- No. of replicates per control: 1
GROWTH CONDITIONS
- Photoperiod: complete darkness
- Day/night temperatures: 25 ± 1 °C
- Relative humidity (%): 70 ± 5%
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
For each test species the number of seeds that germinate was counted and root lengths were measured after 7 days.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Because no significant phytotoxicity was showed in the range-finding test (p<0.05), no definitive testing was carried out. - Nominal and measured concentrations:
- Nominal: 0, 10, 100, 1000 mg/L
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Lycopersicon esculentum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Cucumis sativus
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Lactuca sativa
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Glycine max (G. soja)
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Brassica oleracea var. capitata
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Triticum aestivum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- other: Vigna radiate
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- other: Sesamum indicum
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Daucus carota
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Species:
- Zea mays
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination and root length
- Details on results:
- SEED GERMINATION
- Percent seed germination: No relation between seeds germination and the test item at different concentrations was observed.
- Root length: Root lengthes between control and treatment groups showed no significant difference at the 0.05 level.
- Other effects: At the end of the test, more than 65% of the control seeds germinated and the roots developed were longer than 20 mm. - Reported statistics and error estimates:
- The mean root length for each concentration were compared with the control mean using an multiple comparison method of Dunnett´s tests (ANOVA, SPSS 17.0).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
