Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Pseudomonas putida

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 Apr 1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)

Version / remarks:

1992

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the required amount of test item was directly added to test vessel.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida, Migula, Berlin 33/2 (DSM 50026)
- Source: VTB microbiology, Henkel KGaA

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

21.0 - 21.1 °C

Nominal and measured concentrations:

Nominal: 30, 100, 300, 1000 and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flask
- Fill volume: 100 mL
- Aeration: yes
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to German standard DIN 38412, part 27, section 8.1

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

3 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Reported statistics and error estimates:

Linear regression (Probit Analysis)

Table 1: Inhibition of O2-consumption

Concentration [mg prod./L]	Inhibition (mean value) [%]
30	3.1
100	8.5
300	13.8
1000	34.4
10000	67.9

 

Conclusions:

The EC50 was 3200 mg/L for Pseudomonas putida.

Description of key information

EC50 (30 min): 3200 mg/L for Pseudomonas putida (DIN 38412-27)

Additional information

One study investigating the toxicity to microorganisms of Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters (CAS 92044-91-2) according to DIN 38412-27 is available. The test was conducted with Pseudomonas putida as test organisms. P. putida were exposed to nominal test concentrations of 30, 100, 300, 1000 and 10000 mg/Lfor 30 minutes. Based on the oxygen consubtion of the bacteria an EC50 (30 min) of 3200 mg/L (EC10 (30 min): 140 mg/L) based on nominal concentrations was determined.