Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 Apr - 07 May 1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1, 100, 200, 400 mg/L (nominal - loading rate)

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The required amount of test substance was directly addd to the test medium and stirred for 10 sec with the use of an Ultraturrax
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): high turbidity increasing with increasing test substance concentration

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zabra fish
- Source: Bio International

ACCLIMATION
- Acclimation period: not given
- Type and amount of food during acclimation: DELTA Bio; Fish I
- Health during acclimation (any mortality observed): <5% during the last week before test start


FEEDING DURING TEST
no feeding during test

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Test 1: 21.2 - 21.4 °C
Test 2: 20.4 - 21.0 °C

pH:

Test 1: 7.1 - 7.9
Test 2: 7.2 - 8.2

Dissolved oxygen:

Test 1: 72%
Test 2: 81%

Nominal and measured concentrations:

Nominal loading rate Test 1: 1, 100 mg/L
Nominal loading rate Test 2: 200, 400, 800 mg/L

Mearued: see table below

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L aquarium
- Fill volume: 5L
- Renewal rate of test solution: every 24 h
- Aeration: yes
- No. of organisms per vessel: 10 (3 at 800 mg/L)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: Mortalities and symptoms of toxicity were recorded at at 2-4, 24, 48, 72 and 96 hours test duration.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

65 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

260 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: not observed

Validity criteria fulfilled:

not specified

Description of key information

LC50 (96 h): 260 mg/L (nominal, loading rate), Danio rerio (OECD 203)

Additional information

One study investigating the acute toxicity of Fatty acids, C8-10, oxybis(2-hydroxy-3,1-propanediyl) esters (CAS 92044-91-2) to freshwater fish according to OECD guideline 203 is available (GLP). The study was set up in a semi-static test system with Zebra fish (Danio rerio) as test organisms. The fish were exposed to the water accommodated fractions (WAF) with nominal loading rates of 1, 100, 200, 400 and 800 mg/L. The test substance concentrations were analytically verified by GC analysis. The recovery was 64.5% for the nominal concentration of 100 mg/L. For the nominal concentrations of 200/400 mg/L a recovery rate of and 101.9% was determined. At test termination after 96 hours a LC50 of 260 mg/L (LC0: 65.0 mg/L) based on the nominal loading rate (WAF) was determined.