[2-(acryloyloxy)ethyl]trimethylammonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05/12/1994-09/12/1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

EC Number:

256-176-6

EC Name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

Cas Number:

44992-01-0

Molecular formula:

C8H16NO2.Cl

IUPAC Name:

Trimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride

Details on test material:

- Name of test material: ADAM MECL
- Physical state: slightly viscous and colourless liquid
- Storage condition of test material: in darkness and at room temperature
- quantity received: 250 mL
- Identification number for the study: 94097

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test medium consisted of a mixture of experimental water and test substance at the selected concentrations. A stock solution of 1000mg/l was prepared approximately 45 minutes prior to the start of the study by manually adding the test substance into dilution water. This stock solution was submitted to magnetic stirring for 10 minutes. The final stock solution was homogeneous and limpid. The pH was not adjusted before or during the test.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish

No food was provided during the study
Number of fish per group at the start of the study: 10

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

90 mg of CaCO3 per litre

Test temperature:

19.5 - 21.0 °C

pH:

6.42 - 6.98

Dissolved oxygen:

72 - 99% of the air saturation value throughout the test.

Nominal and measured concentrations:

0, 1, 10 and 100 mg/L

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse osmosis natural water (from a depth of 60m) with added salts to obtain the required characteristics.
- Particulate matter: no
- Metals: no
- Pesticides: no
- Chlorine: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 2 hours, then every 24 hrs
TEST CONCENTRATIONS
- Spacing factor for test concentrations: log 10
- Range finding study
- Test concentrations: 0, 1, 10 and 100 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

There were no mortalities at any of the test concentrations or in the control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the test, the test substance is not harmful to freshwater fish at a concentration of 100 mg/l.