[(dimethylamino)methyl]phenol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-10-02 to 2019-02-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 19.8 - 29.6 - 44.4 - 66.7 - 100 mg/L
- Sampling method: At the start of the exposure (0 hours), samples were taken after preparation of the test item concentrations and analyzed within the same day. At the end of the exposure (48 hours), samples of the 48-hours aged media were taken from the test vessels.
- Sample storage conditions before analysis: The samples were stored at room temperature

Vehicle:

no

Details on test solutions:

- Method: A stock solution (200 mg/L of the test item were weighed out) was freshly prepared with dilution water (M4 medium according to OECD202) prior to the start of the exposure (at 0 hours). The stock solution was shaken manually at room temperature. 5 test item concentrations: 19.8 - 29.6 - 44.4 - 66.7 - 100 mg/L in a geometric series with a separation factor of 1.5 was prepared by dilution of the stock solution 200 mg/L with dilution water.
- Eluate: no
- Differential loading: no
- Controls: M4 medium according to OECD202
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): no vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no undissolved material observed

Test organisms (species):

Daphnia magna

Details on test organisms:

Test system Daphnia magna STRAUS, obtained from continuous laboratory cultures.

Reason for the selection Daphnia magna is the preferred species in accordance with the
of the test system test guideline and is bred at the test facility.

Origin Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany

Breeder Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany

Culture In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20  2 °C, in an incubator, 16 hours illumination; light intensity of max. 1500 lx

Culture medium Elendt M4, according to OECD 202, Annex 3 (2004) is used. The composition of the culture medium is presented in Table 2.

Culture feeding The culture daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus. The algae were cultured at the test facility.

Age of parental stock (mean and range, SD): Less than 24 hours old daphnids from a healthy stock

Feeding during test: The daphnids were not fed during the study.

Acclimation period: Acclimation of the daphnids was not necessary, because the dilution water was equivalent to the culture medium

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Dilution water
298 mgCaC03/L

Test temperature:

18 - 22 °C, constant within ± 1 °C

pH:

Control
- t(0h) = 7.47
- t(48h) = 7.32
100 mg/L
- t(0h) = 8.64
- t(48h) = 7.43

Dissolved oxygen:

Dilution water:
9.06 mg/L
Control
- t(0h) = 9.06 mg/l
- t(48h) = 8.11 mg/L
100 mg/L
- t(0h) = 8.91mg/l
- t(48h) = 8.00 mg/L

Conductivity:

Dilution water
628 [µS/cm]

Nominal and measured concentrations:

Nominal concentration measured concentration
0 h:
Control < LOQ < LOQ (0.4 mg/L)
100 mg/L 101 mg/L
19.8 mg/L 20.1mg/L
48 h:
Control < LOQ < LOQ
100 mg/L 93.2 mg/L
19.8 mg/L 19.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL
- Type (delete if not applicable): Loosely covered with watch glasses
- Material, size, headspace, fill volume: 20 mL
- Aeration: No
- No. of organisms per vessel: 20 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to OECD202
- Total organic carbon: 298 [mg CaCO3/L]
- Alkalinity: pH= 9.06
- Conductivity: 628 [µS/cm]
- Culture medium different from test medium: No
- Intervals of water quality measurement: At the start (t= 0h) and at the end (t= 48h) of the test.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility/mortality; after 24 and 48 hours.
VEHICLE CONTROL PERFORMED: No vehicle was involved
RANGE-FINDING STUDY: Yes
- Test concentrations: 0.5; 5 and 50 mg/L prepared with dilution water
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

73 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

66.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Behavioural abnormalities: No
- Observations on body length and weight: No
- Other biological observations: No
- Mortality of control: No
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No

Results with reference substance (positive control):

- Results with reference substance valid: Yes
- Relevant effect levels: EC50 (24 h)
- Limit test: no
- Dose-response test: yes
- EC50 (24h): 2.09 mg/l (CI; 1.00 – 4.00)
- Other: Valid range according to AQS P9/2 (02/2000), clone 5 = 0.6 – 2.4 mg/l
Valid range according to OECD202 (2004), clone A = 0.6 – 2.1 mg/l

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations of the test item, the 48 hour-EC50 for Daphnia magna was 73.0 mg/L (confidence limits: 67.0 – 93.4 mg/L).

Executive summary:

The toxicity of the test item to Daphnia magna(STRAUS) was determined according to OECD 202 (2004). The study was conducted under static conditions over a period of 48 hours with 5 test concentrations in the range of 19.8 - 100 mg/L, prepared in a geometric series with a separation factor of 1.5. All tested concentration levels were visually clear throughout the exposure period.Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration leveland the control. The concentrations of the test item were analytically verified via HPLC-DAD at the start (0 hours) and at the end of the exposure (48 hours) in all concentration levels and in the control. The measured concentrations of the test item were in the range of 97 to 103% of the nominal values at the start of the exposure (0 hours) and 93 to 99% of the nominal values at the end of the exposure (48 hours). The measured test item concentrations were all within ±20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained during the whole duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item. The 48 hour-EC50 value of the test item on Daphnia magna was 73.0 mg/L (confidence limits: 67.0 – 93.4 mg/L). The validity criteria of the test guideline were fulfilled.

Description of key information

The toxicity of the test item to Daphnia magna (STRAUS) was determined according to OECD 202 (2004).The study was conducted under static conditions over a period of 48 hours with 5 test concentrations in the range of 19.8 - 100 mg/L, prepared in a geometric series with a separation factor of 1.5. All tested concentration levels were visually clear throughout the exposure period.Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration leveland the control.The concentrations of the test item were analytically verified via HPLC-DAD at the start (0 hours) and at the end of the exposure (48 hours) in all concentration levels and in the control.The measured concentrations of the test item were in the range of 97 to 103% of the nominal values at the start of the exposure (0 hours) and 93 to 99% of the nominal values at the end of the exposure (48 hours).The measured test item concentrations were all within±20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained during the whole duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item.The 48 hour-EC50 value of the test item on Daphnia magnawas 73.0 mg/L (confidence limits: 67.0 – 93.4 mg/L).The validity criteria of the test guideline were fulfilled.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

73 mg/L