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EC number: 224-618-7 | CAS number: 4430-18-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The calculated mean biodegradation value was 4 % of the added total organic carbon (TOC) at the end of the test. Consequently, the test item was not readily biodegradable under the test conditions within 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The test item was investigated for its ready biodegradability in a 28-Day CO2 Evolution (Modified Sturm) Test according to the OECD Guideline for Testing of Chemicals, No. 301 B (1992), the Method C.4-C of Commission Regulation (EC) No 440/2008 and the US EPA OPPTS 835.3110 Ready Biodegradability, Paragraph (m) (1998) and following GLP.
The test item was added to the test vessels at a final test concentration of 35.0 mg/L, corresponding to an added carbon content of 15.7 mg/L. The inoculum (activated sludge) was added to the test vessels at a final concentration of 30 mg dry material per liter. The CO2 evolved from the test vessels containing the test item in inoculated mineral medium was in the range of the inoculum controls throughout the exposure period of 28 days. The calculated mean biodegradation value was 4 % of the added total organic carbon (TOC) at the end of the test. Consequently, the test item was not readily biodegradable under the test conditions within 28 days. In the toxicity control, containing both the test item (15.7 mg TOC/L) and the reference item sodium benzoate (15.0 mg TOC/L), biodegradation reached 45 % of the overall added TOC within 14 days of exposure. Thus, the test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 35.0 mg/L. In the procedure controls, average biodegradation of the reference item was 91 % by Exposure Day 14, thus confirming suitability of the activated sludge (≥ 60 % degradation by Exposure Day 14). By the end of the test (Exposure Day 28), average biodegradation was 95 %. The results are considered to be valid since the following criteria are met:
- The percentage degradation of the reference item reached with 76 % by Exposure Day 5 the pass level for ready biodegradability (criterion: at least 60 % in a 10-day window by Day 14).
- The total CO2 evolution in the inoculum controls at the end of the test was 26 mg CO2/L for both replicates (corresponding to 21 mg C per vessel; criterion: less than 40 mg CO2/L, corresponding to 33 mg C per vessel).
- The IC content in the test item vessels was less than 0.1 % of the TC (criterion: less than 5 % of the TC).
Since the test item was not degraded, other validity criteria are not applicable.
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